BMRB Entry 51783

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51783

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Title:
Chemical Shift Assignments of Retinal Guanylyl Cyclase Activating Protein 5 (GCAP5) with a Mutation (R22A) that Abolishes Dimerization and Enhances Cyclase Activation
Deposition date:
2023-01-10
Original release date:
2023-05-24
Authors:
Cudia, Diana; Ahoulou, Effibe; Ames, James
Citation:

Citation: Cudia, Diana; Ahoulou, Effibe; Ames, James. "Chemical shift assignments of retinal guanylyl cyclase activating protein 5 (GCAP5) with a mutation (R22A) that abolishes dimerization and enhances cyclase activation"  Biomol. NMR Assignments 17, 115-119 (2023).
PubMed: 37129703

Assembly members:

Assembly members:
entity_1, polymer, 198 residues, Formula weight is not available
entity_MYR, non-polymer, 228.371 Da.

Natural source:

Natural source:   Common Name: zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDSSSMSATELSACKCHQW YAKFMTECPSGQLTFYEFKK FFGLKNLSEKSNAYVNTMFK TFDIDDDGCIDFMEYVAALS LVLKGGVQQKLRWYFKLFDM DGSGCIDKDELLLIFKAVQA INGAEPEISAEDLADIVFNK IDVNGDGELSLEEFMEGISA DEKISEMLTQSLDLTRIVSN IYNDSYIEQEAEIIEDQA

Data sets:
Data typeCount
13C chemical shifts678
15N chemical shifts191
1H chemical shifts965

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GCAP5 R22A1
2myristic acid2

Entities:

Entity 1, GCAP5 R22A 198 residues - Formula weight is not available

1   METGLYASPSERSERSERMETSERALATHR
2   GLULEUSERALACYSLYSCYSHISGLNTRP
3   TYRALALYSPHEMETTHRGLUCYSPROSER
4   GLYGLNLEUTHRPHETYRGLUPHELYSLYS
5   PHEPHEGLYLEULYSASNLEUSERGLULYS
6   SERASNALATYRVALASNTHRMETPHELYS
7   THRPHEASPILEASPASPASPGLYCYSILE
8   ASPPHEMETGLUTYRVALALAALALEUSER
9   LEUVALLEULYSGLYGLYVALGLNGLNLYS
10   LEUARGTRPTYRPHELYSLEUPHEASPMET
11   ASPGLYSERGLYCYSILEASPLYSASPGLU
12   LEULEULEUILEPHELYSALAVALGLNALA
13   ILEASNGLYALAGLUPROGLUILESERALA
14   GLUASPLEUALAASPILEVALPHEASNLYS
15   ILEASPVALASNGLYASPGLYGLULEUSER
16   LEUGLUGLUPHEMETGLUGLYILESERALA
17   ASPGLULYSILESERGLUMETLEUTHRGLN
18   SERLEUASPLEUTHRARGILEVALSERASN
19   ILETYRASNASPSERTYRILEGLUGLNGLU
20   ALAGLUILEILEGLUASPGLNALA

Entity 2, myristic acid - C14 H28 O2 - 228.371 Da.

1   MYR

Samples:

sample_1: GCAP5 R22A, [U-99% 15N], 0.75 mM; TRIS-d11, [U-99% 2H], 5 mM; DTT-d10, [U-99% 2H], 2 mM

sample_2: GCAP5 R22A, [U-99% 13C; U-99% 15N], 0.75 mM; TRIS-d11, [U-99% 2H], 5 mM; DTT-d10, [U-99% 2H], 2 mM

sample_3: GCAP5 R22A, [U-99% 13C; U-99% 15N], 0.75 mM; TRIS-d11, [U-99% 2H], 5 mM; DTT-d10, [U-99% 2H], 2 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 305 K

sample_conditions_2: pH: 7.4; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCACBisample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOCACBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HBHANHsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D HBCBCGCDCEHEsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks