BMRB Entry 51771
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51771
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Citation: Ohtomo, Hideaki; Yamane, Tsutomu; Oda, Takashi; Kodera, Noriyuki; Kurita, Junichi; Tsunaka, Yasuo; Amyot, Romain; Ikeguchi, Mutsunori; Nishimura, Yoshifumi. "Dynamic solution structures of whole human NAP1 dimer bound to one and two histone H2A-H2B heterodimers obtained by integrative methods" J. Mol. Biol. 435, 168189-168189 (2023).
PubMed: 37380014
Assembly members:
entity_1, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
entity_1: LGSEEEGDEENDPDYDPKKD
QNPAE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 22 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CTADC | 1 |
Entities:
Entity 1, CTADC 25 residues - Formula weight is not available
| 1 | LEU | GLY | SER | GLU | GLU | GLU | GLY | ASP | GLU | GLU | ||||
| 2 | ASN | ASP | PRO | ASP | TYR | ASP | PRO | LYS | LYS | ASP | ||||
| 3 | GLN | ASN | PRO | ALA | GLU |
Samples:
sample_1: CTADC of NAP1, [U-99% 15N], 475 uM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
Olivia - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
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