BMRB Entry 5177

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PDB ID: 1tvj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5177
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for chick cofilin

Deposition date:

2001-10-12

Original release date:

2002-05-06

Authors:

Bains, Naresh; Gorbatyuk, Vitaliy; Nosworthy, Neil; Robson, Scott; Maciejewski, Mark; dos Remedios, Cristobal; King, Glenn

Citation:

Citation: Bains, Naresh; Gorbatyuk, Vitaliy; Nosworthy, Neil; Robson, Scott; Maciejewski, Mark; dos Remedios, Cristobal; King, Glenn. "Letter to the Editor: Backbone and Side-chain 1H, 15N, and 13C Assignments for Chick Cofilin" J. Biomol. NMR 22, 193-194 (2002).
PubMed: 11885570

Assembly members:

Assembly members:
chick cofilin, polymer, 166 residues, 18662 Da.

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chick cofilin: MASGVTVNDEVIKVFNDMKV RKSSTPEEIKKRKKAVLFCL SDDKKQIIVEEAKQILVGDI GDTVEDPYTAFVKLLPLNDC RYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASS KDAIKKKFTGIKHEWQVNGL DDIKDRSTLGEKLGGNVVVS LEGKPL

Data sets:

assigned_chemical_shifts
- Cofilin_chemical_shifts

Data type	Count
1H chemical shifts	1233
13C chemical shifts	713
15N chemical shifts	172

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	chick cofilin	1

Entities:

Entity 1, chick cofilin 166 residues - 18662 Da.

1

MET

ALA

SER

GLY

VAL

THR

VAL

ASN

ASP

GLU

2

VAL

ILE

LYS

VAL

PHE

ASN

ASP

MET

LYS

VAL

3

ARG

LYS

SER

THR

PRO

GLU

ILE

LYS

4

LYS

ARG

LYS

ALA

VAL

LEU

PHE

CYS

LEU

5

SER

ASP

LYS

GLN

ILE

VAL

GLU

6

GLU

ALA

LYS

GLN

ILE

LEU

VAL

GLY

ASP

ILE

7

GLY

ASP

THR

VAL

GLU

ASP

PRO

TYR

THR

ALA

8

PHE

VAL

LYS

LEU

PRO

LEU

ASN

ASP

CYS

9

ARG

TYR

ALA

LEU

TYR

ASP

ALA

THR

TYR

GLU

10

THR

LYS

GLU

SER

LYS

GLU

ASP

LEU

VAL

11

PHE

ILE

PHE

TRP

ALA

PRO

GLU

SER

ALA

PRO

12

LEU

LYS

SER

LYS

MET

ILE

TYR

ALA

SER

13

LYS

ASP

ALA

ILE

LYS

PHE

THR

GLY

14

ILE

LYS

HIS

GLU

TRP

GLN

VAL

ASN

GLY

LEU

15

ASP

ILE

LYS

ASP

ARG

SER

THR

LEU

GLY

16

GLU

LYS

LEU

GLY

ASN

VAL

SER

17

LEU

GLU

GLY

LYS

PRO

LEU

Samples:

Sample_1: chick cofilin, [U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; H2O 92.5%; D2O 7.5%

Sample_2: chick cofilin, [U-99% 13C; U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; H2O 92.5%; D2O 7.5%

Sample_3: chick cofilin, [U-99% 13C; U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; D2O 100%

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.06 M

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	not available	not available	Ex-cond_1
3D CBCA(CO)NH	not available	not available	Ex-cond_1
3D HNCO	not available	not available	Ex-cond_1
3D HCCH-COSY	not available	not available	Ex-cond_1
3D HCCH-TOCSY	not available	not available	Ex-cond_1
3D HC(CO)NH-TOCSY	not available	not available	Ex-cond_1
3D C(CO)NH-TOCSY	not available	not available	Ex-cond_1
3D 15N-edited NOESY-HSQC	not available	not available	Ex-cond_1
3D 13C-edited NOESY-HSQC	not available	not available	Ex-cond_1
2D (HB)CB(CGCD)HD	not available	not available	Ex-cond_1
2D 1H-1H NOESY	not available	not available	Ex-cond_1
2D 1H-1H DQF-COSY	not available	not available	Ex-cond_1
2D TOCSY	not available	not available	Ex-cond_1

Software:

nmrPipe - NMR data processing

XEASY - Spectral analysis, resonance assignment

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	1TVJ
DBJ	BAE27737 BAE87142 BAG36842
GB	AAA37433 AAA62732 AAD31280 AAD31281 AAF64498
REF	NP_001004406 NP_001020386 NP_001069622 NP_001102452 NP_001162212
SP	P21566 P45591 Q148F1 Q5G6V9 Q9Y281
TPG	DAA17465
AlphaFold	P21566 P45591 Q148F1 Q5G6V9 Q9Y281