BMRB Entry 51769

Title:
Chemical shifts of UBQLN1 514-589, F547Y at 50 micromolar
Deposition date:
2023-01-09
Original release date:
2023-01-30
Authors:
Buel, Gwen; Chen, Xiang; Walters, Kylie
Citation:

Citation: Buel, Gwen; Chen, Xiang; Kayode, Olumide; Cruz, Anthony; Walters, Kylie. "1H, 15N, 13C backbone and Cb resonance assignments for UBQLN1 UBA and UBAA domains"  Biomol. NMR Assignments 17, 101-106 (2023).
PubMed: 37022617

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts71
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBQLN1 514-589, F547Y1

Entities:

Entity 1, UBQLN1 514-589, F547Y 76 residues - Formula weight is not available

1   THRALAGLYTHRTHRGLUPROGLYHISGLN
2   GLNPHEILEGLNGLNMETLEUGLNALALEU
3   ALAGLYVALASNPROGLNLEUGLNASNPRO
4   GLUVALARGTYRGLNGLNGLNLEUGLUGLN
5   LEUSERALAMETGLYPHELEUASNARGGLU
6   ALAASNLEUGLNALALEUILEALATHRGLY
7   GLYASPILEASNALAALAILEGLUARGLEU
8   LEUGLYSERGLNPROSER

Samples:

sample_1: UBQLN1 514-589, F547Y, [U-100% 15N], 50 uM; MOPS 10 mM; NaCl 50 mM; DTT 5 mM; zinc sulfate 10 uM; pefabloc 1 mM; NaN3 0.1%

sample_conditions_1: ionic strength: 60 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

XEASY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks