BMRB Entry 51769

Name: Chemical shifts of UBQLN1 514-589, F547Y at 50 micromolar
Published: 2023-01-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51769

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts of UBQLN1 514-589, F547Y at 50 micromolar

Deposition date:

2023-01-09

Original release date:

2023-01-30

Authors:

Buel, Gwen; Chen, Xiang; Walters, Kylie

Citation:

Citation: Buel, Gwen; Chen, Xiang; Kayode, Olumide; Cruz, Anthony; Walters, Kylie. "1H, 15N, 13C backbone and Cb resonance assignments for UBQLN1 UBA and UBAA domains" Biomol. NMR Assignments 17, 101-106 (2023).
PubMed: 37022617

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TAGTTEPGHQQFIQQMLQAL AGVNPQLQNPEVRYQQQLEQ LSAMGFLNREANLQALIATG GDINAAIERLLGSQPS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	71
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBQLN1 514-589, F547Y	1

Entities:

Entity 1, UBQLN1 514-589, F547Y 76 residues - Formula weight is not available

1

THR

ALA

GLY

THR

GLU

PRO

GLY

HIS

GLN

2

GLN

PHE

ILE

GLN

MET

LEU

GLN

ALA

LEU

3

ALA

GLY

VAL

ASN

PRO

GLN

LEU

GLN

ASN

PRO

4

GLU

VAL

ARG

TYR

GLN

LEU

GLU

GLN

5

LEU

SER

ALA

MET

GLY

PHE

LEU

ASN

ARG

GLU

6

ALA

ASN

LEU

GLN

ALA

LEU

ILE

ALA

THR

GLY

7

GLY

ASP

ILE

ASN

ALA

ILE

GLU

ARG

LEU

8

LEU

GLY

SER

GLN

PRO

SER

Samples:

sample_1: UBQLN1 514-589, F547Y, [U-100% 15N], 50 uM; MOPS 10 mM; NaCl 50 mM; DTT 5 mM; zinc sulfate 10 uM; pefabloc 1 mM; NaN3 0.1%

sample_conditions_1: ionic strength: 60 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

XEASY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks