BMRB Entry 51763

Name: MITF C-terminus backbone assignments
Published: 2023-08-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51763

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MITF C-terminus backbone assignments

Deposition date:

2023-01-06

Original release date:

2023-08-31

Authors:

Langelaan, David; Brown, Alexandra; Lynch, Kyle

Citation:

Citation: Brown, Alexandra; Lynch, Kyle; Langelaan, David. "Structural analysis of the interactions between the C-terminal transactivation domain of MITF and the TAZ2 domain of CBP/p300" Biochemistry ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSEMQARAHGLSLIPSTGLC SPDLVNRIIKQEPVLENCSQ DLLQHHADLTCTTTLDLTDG TITFNNNLGTGTEANQAYSV PTKMGSKLEDILMDDTLSPV GVTDPLLSSVSPGASKTSSR RSSMSMEETEHTC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	361
15N chemical shifts	116
1H chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MITF	1

Entities:

Entity 1, MITF 133 residues - Formula weight is not available

1

GLY

SER

GLU

MET

GLN

ALA

ARG

ALA

HIS

GLY

2

LEU

SER

LEU

ILE

PRO

SER

THR

GLY

LEU

CYS

3

SER

PRO

ASP

LEU

VAL

ASN

ARG

ILE

LYS

4

GLN

GLU

PRO

VAL

LEU

GLU

ASN

CYS

SER

GLN

5

ASP

LEU

GLN

HIS

ALA

ASP

LEU

THR

6

CYS

THR

LEU

ASP

LEU

THR

ASP

GLY

7

THR

ILE

THR

PHE

ASN

LEU

GLY

THR

8

GLY

THR

GLU

ALA

ASN

GLN

ALA

TYR

SER

VAL

9

PRO

THR

LYS

MET

GLY

SER

LYS

LEU

GLU

ASP

10

ILE

LEU

MET

ASP

THR

LEU

SER

PRO

VAL

11

GLY

VAL

THR

ASP

PRO

LEU

SER

VAL

12

SER

PRO

GLY

ALA

SER

LYS

THR

SER

ARG

13

ARG

SER

MET

SER

MET

GLU

THR

GLU

14

HIS

THR

CYS

Samples:

sample_1: MITF289-419, [U-99% 13C; U-99% 15N], 700 uM; D2O 5%; DTT 5 mM; MES 20 mM; NaCl 25 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks