BMRB Entry 51758

Title:
Oct4_aa286-360_tev
Deposition date:
2023-01-03
Original release date:
2023-01-05
Authors:
Theillet, Francois-Xavier
Citation:

Citation: Theillet, Francois-Xavier. "Structural characterization of Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to identify their phospho-dependent binding partners."  C. R. Chim. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-41b(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts68
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Oct4_aa286-360-tev1

Entities:

Entity 1, Oct4_aa286-360-tev 82 residues - Formula weight is not available

1   GLYLYSARGSERSERSERASPTYRALAGLN
2   ARGGLUASPPHEGLUALAALAGLYSERPRO
3   PHESERGLYGLYPROVALSERPHEPROLEU
4   ALAPROGLYPROHISPHEGLYTHRPROGLY
5   TYRGLYSERPROHISPHETHRALALEUTYR
6   SERSERVALPROPHEPROGLUGLYGLUALA
7   PHEPROPROVALSERVALTHRTHRLEUGLY
8   SERPROMETHISSERASNGLUASNLEUTYR
9   PHEGLN

Samples:

sample_1: entity_1, [U-98% 13C; U-98% 15N], 500 ± 100 uM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.1 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(NCA)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.1 - chemical shift assignment

TOPSPIN v3 - collection, processing

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks