BMRB Entry 51750

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51750

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Title:
Myocilin Olfactomedin Domain Backbone Assignment
Deposition date:
2022-12-21
Original release date:
2025-09-26
Authors:
Saccuzzo, Emily; Mebrat, Mubark; Scelsi, Hailee; Kim, Minjoo; Thua Ma, Minh; Hill, Shannon; Gumbart, J.C; Van Horn, Wade; Lieberman, Raquel
Citation:

Citation: Saccuzzo, Emily; Mebrat, Mubark; Scelsi, Hailee; Kim, Minjoo; Thua Ma, Minh; Hill, Shannon; Gumbart, J.C; Van Horn, Wade; Lieberman, Raquel. "Myocilin Olfactomedin Domain Backbone Assignment"  .

Assembly members:

Assembly members:
entity_1, polymer, 277 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
entity_NA, non-polymer, 22.990 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL-c4x

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LKESPSGYLRSGEGDTGCGE LVWVGEPLTLRTAETITGKY GVWMRDPKPTYPYTQETTWR IDTVGTDVRQVFEYDLISQF MQGYPSKVHILPRPLESTGA VVYSGSLYFQGAESRTVIRY ELNTETVKAEKEIPGAGYHG QFPYSWGGYTDIDLAVDEAG LWVIYSTDEAKGAIVLSKLN PENLELEQTWETNIRKQSVA NAFIICGTLYTVSSYTSADA TVNFAYDTGTGISKTLTIPF KNRYKYSSMIDYNPLEKKLF AWDNLNMVTYDIKLSKM

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts152
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myocilin olfactomedin domain1
2Calcium2
3Sodium3

Entities:

Entity 1, myocilin olfactomedin domain 277 residues - Formula weight is not available

1   LEULYSGLUSERPROSERGLYTYRLEUARG
2   SERGLYGLUGLYASPTHRGLYCYSGLYGLU
3   LEUVALTRPVALGLYGLUPROLEUTHRLEU
4   ARGTHRALAGLUTHRILETHRGLYLYSTYR
5   GLYVALTRPMETARGASPPROLYSPROTHR
6   TYRPROTYRTHRGLNGLUTHRTHRTRPARG
7   ILEASPTHRVALGLYTHRASPVALARGGLN
8   VALPHEGLUTYRASPLEUILESERGLNPHE
9   METGLNGLYTYRPROSERLYSVALHISILE
10   LEUPROARGPROLEUGLUSERTHRGLYALA
11   VALVALTYRSERGLYSERLEUTYRPHEGLN
12   GLYALAGLUSERARGTHRVALILEARGTYR
13   GLULEUASNTHRGLUTHRVALLYSALAGLU
14   LYSGLUILEPROGLYALAGLYTYRHISGLY
15   GLNPHEPROTYRSERTRPGLYGLYTYRTHR
16   ASPILEASPLEUALAVALASPGLUALAGLY
17   LEUTRPVALILETYRSERTHRASPGLUALA
18   LYSGLYALAILEVALLEUSERLYSLEUASN
19   PROGLUASNLEUGLULEUGLUGLNTHRTRP
20   GLUTHRASNILEARGLYSGLNSERVALALA
21   ASNALAPHEILEILECYSGLYTHRLEUTYR
22   THRVALSERSERTYRTHRSERALAASPALA
23   THRVALASNPHEALATYRASPTHRGLYTHR
24   GLYILESERLYSTHRLEUTHRILEPROPHE
25   LYSASNARGTYRLYSTYRSERSERMETILE
26   ASPTYRASNPROLEUGLULYSLYSLEUPHE
27   ALATRPASPASNLEUASNMETVALTHRTYR
28   ASPILELYSLEUSERLYSMET

Entity 2, Calcium - Ca - 40.078 Da.

1   CA

Entity 3, Sodium - Na - 22.990 Da.

1   NA

Samples:

sample_1: myocilin olfactomedin domain, [U-98% 13C; U-98% 15N], 570 uM; Na2HP04 25 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 0.075 M; pH: 6.8; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CcpNMR v3.0.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks