BMRB Entry 51749

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51749

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Title:
Backbone resonance, Cb, and ILV-CH3 assignments of human Atg3 with deletion of 90 to 190 residues and H240Y, V241A, P263G, and H266L mutations in bicelles
Deposition date:
2022-12-20
Original release date:
2023-07-27
Authors:
Ye, Yansheng; Tian, Fang
Citation:

Citation: Ye, Yansheng; Tyndall, Erin; Bui, Van; Bewley, Maria; Wang, Guifang; Hong, Xupeng; Shen, Yan; Flanagan, John; Wang, HongGang; Tian, Fang. "Translating Membrane Geometry into Protein Function: Multifaceted Membrane Interactions of Human Atg3 to Promote LC3-Phosphatidylethanolamine Conjugation during Autophagy"  .

Assembly members:

Assembly members:
entity_1, polymer, 213 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQNVINTVKGKALEVAEYLT PVLKESKFKETGVITPEEFV AAGDHLVHHCPTWQWATGEE LKVKAYLPTGKQFLVTKNVP CYKRCKQMEEDAILQTRTYD LYITYDKYYQTPRLWLFGYD EQRQPLTVEHMYEDISQDYA KKTVTIENHPHLPPPPMCSV HGCRLAEVMKKIIETVAEGG GELGVHMYLLIFLKFVQAVI PTIEYDYTRHFTM

Data sets:
Data typeCount
13C chemical shifts641
15N chemical shifts188
1H chemical shifts437

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hAtg31

Entities:

Entity 1, hAtg3 213 residues - Formula weight is not available

1   METGLNASNVALILEASNTHRVALLYSGLY
2   LYSALALEUGLUVALALAGLUTYRLEUTHR
3   PROVALLEULYSGLUSERLYSPHELYSGLU
4   THRGLYVALILETHRPROGLUGLUPHEVAL
5   ALAALAGLYASPHISLEUVALHISHISCYS
6   PROTHRTRPGLNTRPALATHRGLYGLUGLU
7   LEULYSVALLYSALATYRLEUPROTHRGLY
8   LYSGLNPHELEUVALTHRLYSASNVALPRO
9   CYSTYRLYSARGCYSLYSGLNMETGLUGLU
10   ASPALAILELEUGLNTHRARGTHRTYRASP
11   LEUTYRILETHRTYRASPLYSTYRTYRGLN
12   THRPROARGLEUTRPLEUPHEGLYTYRASP
13   GLUGLNARGGLNPROLEUTHRVALGLUHIS
14   METTYRGLUASPILESERGLNASPTYRALA
15   LYSLYSTHRVALTHRILEGLUASNHISPRO
16   HISLEUPROPROPROPROMETCYSSERVAL
17   HISGLYCYSARGLEUALAGLUVALMETLYS
18   LYSILEILEGLUTHRVALALAGLUGLYGLY
19   GLYGLULEUGLYVALHISMETTYRLEULEU
20   ILEPHELEULYSPHEVALGLNALAVALILE
21   PROTHRILEGLUTYRASPTYRTHRARGHIS
22   PHETHRMET

Samples:

sample_1: human Atg3-delta90_190-4m, [U-13C; U-15N; 2H], 0.5 mM; sodium chloride 150 mM; HEPES 50 mM; TCEP 2 mM

sample_2: human Atg3-delta90_190-4m, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu-13C; 99% 1HG-Val-13C], 0.5 mM; sodium chloride 150 mM; HEPES 50 mM; TCEP 2 mM; DMPC 38.5 mM; DHPC 192.5 mM; DMPG 9.6 mM

sample_3: human Atg3-delta90_190-4m, [U-13C; U-15N; 2H], 0.5 mM; sodium chloride 150 mM; HEPES 50 mM; TCEP 2 mM; DMPC 38.5 mM; DHPC 192.5 mM; DMPG 9.6 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_3isotropicsample_conditions_1
2D 1H-15N TROSYsample_3isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(CO)CACBsample_3isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 15N-separated NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRDraw - processing

NMRView - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCEII 600 MHz
  • Bruker AVANCEIII 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks