BMRB Entry 51739

Name: Backbone 1H, 13C and 15N assignments of the apo-acyl carrier protein of Pseudomonas aeruginosa
Published: 2023-12-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51739

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N assignments of the apo-acyl carrier protein of Pseudomonas aeruginosa

Deposition date:

2022-12-14

Original release date:

2023-12-19

Authors:

Warner, Lisa; Nagarajan, Rajesh; Rizzo, Madison

Citation:

Citation: Rizzo, Madison; Baggs, Eric; Chowdhury, Abu Sayeed; Nagarajan, Rajesh; Warner, Lisa Rose. "Backbone 1H, 13C and 15N assignments of the apo-acyl carrier protein (ACP1) of Pseudomonas aeruginosa" Biomol. NMR Assignm. 17, 183-188 (2023).
PubMed: 37421542

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD441-NH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKHHHHHHMDDIETRVRKLV AARFGVEECDIRLDSDFRND FGAESLEVVELVMALEAEFG VEIADDDAERIETVRQAIDY LEEAVPT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	230
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo-ACP1	1

Entities:

Entity 1, apo-ACP1 87 residues - Formula weight is not available

1

MET

LYS

HIS

MET

ASP

2

ASP

ILE

GLU

THR

ARG

VAL

ARG

LYS

LEU

VAL

3

ALA

ARG

PHE

GLY

VAL

GLU

CYS

ASP

4

ILE

ARG

LEU

ASP

SER

ASP

PHE

ARG

ASN

ASP

5

PHE

GLY

ALA

GLU

SER

LEU

GLU

VAL

GLU

6

LEU

VAL

MET

ALA

LEU

GLU

ALA

GLU

PHE

GLY

7

VAL

GLU

ILE

ALA

ASP

ALA

GLU

ARG

8

ILE

GLU

THR

VAL

ARG

GLN

ALA

ILE

ASP

TYR

9

LEU

GLU

ALA

VAL

PRO

THR

Samples:

sample_1: apo-ACP1, [U-98% 13C; U-98% 15N], 0.9 mM; PO4 40 mM; EDTA 1 mM; DTT 1.25 mM; TSP 0.025 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe vVersion 11.1 - processing

TOPSPIN v3.6.2 - collection

CcpNMR v2.5.2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks