BMRB Entry 51726

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51726
MolProbity Validation Chart

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Title:
1H, 13C and 15N assignments and NOESY peak lists of silver ion-bound SilF from Salmonella typhimurium plasmid pMG101
Deposition date:
2022-12-09
Original release date:
2023-10-20
Authors:
Monneau, Yoan; Hologne, Maggy; Walker, Olivier
Citation:

Citation: Arrault, Cyrielle; Monneau, Yoan Rocky; Martin, Marie; Cantrelle, Francois-Xavier; Boll, Emmanuelle; Chirot, Fabien; Comby Zerbino, Clothilde; Walker, Olivier; Hologne, Maggy. "The battle for silver binding: How the interplay between the SilE, SilF, and SilB proteins contributes to the silver efflux pump mechanism"  J. Biol. Chem. 299, 105004-105004 (2023).
PubMed: 37394004

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, Formula weight is not available
entity_AG, non-polymer, 107.868 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM60

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGMLKHISHGDMNAASDA SVQQVIKGTGIVKDIDMNSK KITISHEAIPAVGWPAMTMR FTFVNADDAINALKTGNHVD FSFIQQGNISLLKSINVTQS

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts75
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SilF1
2silver ion2

Entities:

Entity 1, SilF 100 residues - Formula weight is not available

1   GLYALAMETGLYMETLEULYSHISILESER
2   HISGLYASPMETASNALAALASERASPALA
3   SERVALGLNGLNVALILELYSGLYTHRGLY
4   ILEVALLYSASPILEASPMETASNSERLYS
5   LYSILETHRILESERHISGLUALAILEPRO
6   ALAVALGLYTRPPROALAMETTHRMETARG
7   PHETHRPHEVALASNALAASPASPALAILE
8   ASNALALEULYSTHRGLYASNHISVALASP
9   PHESERPHEILEGLNGLNGLYASNILESER
10   LEULEULYSSERILEASNVALTHRGLNSER

Entity 2, silver ion - Ag - 107.868 Da.

1   AG

Samples:

sample_1: SilF from Salmonella Typhimurium plasmid pMG10, [U-13C; U-15N], 500 uM; Silver ion 500 uM; MES buffer 20 mM; NaCl 100 mM

sample_2: SilF from Salmonella Typhimurium plasmid pMG10 250 uM; Silver ion 250 uM; MES buffer 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D CBCACONHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 15N-separated NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

CYANA - structure solution

CNS - refinement

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks