BMRB Entry 51720

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51720

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Title:
1H, 13C, 15N resonance assignment of intrinsically disordered C-terminal fragment of methoprene tolerant protein (MetC)
Deposition date:
2022-12-05
Original release date:
2023-08-18
Authors:
Bartosinska-Marzec, Paulina; Zawadzka-Kazimierczuk, Anna; Kozminski, Wiktor; Kolonko-Adamska, Marta; Popowicz, Grzegorz; Krezel, Artur; Ozyhar, Andrzej; Greb-Markiewicz, Beata
Citation:

Citation: Kolonko-Adamska, Marta; Zawadzka-Kazimierczuk, Anna; Bartosinska-Marzec, Paulina; Kozminski, Wiktor; Popowicz, Grzegorz; Krezel, Artur; Ozyhar, Andrzej; Greb-Markiewicz, Beata. "Interaction patterns of methoprene-tolerant and germ cell-expressed Drosophila JH receptors suggest significant differences in their functioning"  Front. Mol. Biosci. 10, 1215550-1215550 (2023).
PubMed: 37654797

Assembly members:

Assembly members:
entity_1, polymer, 210 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO (EMBL, Germany)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEAGRQKVQEMKEKFSTIIK AEMPTQSSSPDLPASQAPQQ LERIVLYLIENLQKSVDSAE TVGGQGMESLMDDGYSSPAN TLTLEELAPSPTPALALVPP APSSVKSSISKSVSVVNVTA ARKFQQEHQKQRERDREQLK ERTNSTQGVIRQLSSCLSEA ETASCILSPASSLSASEAPD TPDPHSNTSPPPSLHTRPSV LHRTLTSTLR

Data sets:
Data typeCount
13C chemical shifts502
15N chemical shifts179
1H chemical shifts545

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MetC1

Entities:

Entity 1, MetC 210 residues - Formula weight is not available

1   METGLUALAGLYARGGLNLYSVALGLNGLU
2   METLYSGLULYSPHESERTHRILEILELYS
3   ALAGLUMETPROTHRGLNSERSERSERPRO
4   ASPLEUPROALASERGLNALAPROGLNGLN
5   LEUGLUARGILEVALLEUTYRLEUILEGLU
6   ASNLEUGLNLYSSERVALASPSERALAGLU
7   THRVALGLYGLYGLNGLYMETGLUSERLEU
8   METASPASPGLYTYRSERSERPROALAASN
9   THRLEUTHRLEUGLUGLULEUALAPROSER
10   PROTHRPROALALEUALALEUVALPROPRO
11   ALAPROSERSERVALLYSSERSERILESER
12   LYSSERVALSERVALVALASNVALTHRALA
13   ALAARGLYSPHEGLNGLNGLUHISGLNLYS
14   GLNARGGLUARGASPARGGLUGLNLEULYS
15   GLUARGTHRASNSERTHRGLNGLYVALILE
16   ARGGLNLEUSERSERCYSLEUSERGLUALA
17   GLUTHRALASERCYSILELEUSERPROALA
18   SERSERLEUSERALASERGLUALAPROASP
19   THRPROASPPROHISSERASNTHRSERPRO
20   PROPROSERLEUHISTHRARGPROSERVAL
21   LEUHISARGTHRLEUTHRSERTHRLEUARG

Samples:

sample_1: C terminal fragment of methoprene tolerant protein, [U-98% 13C; U-98% 15N], 450 uM; H2O 90%; D2O, [U-2H], 10%; Bis-Tris 20 mM; NaCl 150 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
5D HN(CA)CONHsample_1isotropicsample_conditions_1
5D (HACA)CON(CA)CONHsample_1isotropicsample_conditions_1
5D HabCabCONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

toastd - processing

cleaner - processing

SPARKY - peak picking

TSAR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks