BMRB Entry 51715

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51715
MolProbity Validation Chart

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NMR-STAR v3 text file.
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All files associated with the entry

Title:
Calmodulin bound to the GluN1 C0 domain of the NMDA receptor
Deposition date:
2022-11-29
Original release date:
2023-03-07
Authors:
Bej, Aritra; Ames, James B.
Citation:

Citation: Bej, Aritra; Ames, James B.. "Chemical shift assignments of calmodulin bound to the GluN1 C0 domain (residues 841-865) of the NMDA receptor"  Biomol. NMR Assign. 17, 61-65 (2023).
PubMed: 36739573

Assembly members:

Assembly members:
entity_1, polymer, 149 residues, Formula weight is not available
entity_2, polymer, 25 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARKMKDT DSEEEIREAFRVFDKDGNGY ISAAELRHVMTNLGEKLTDE EVDEMIREADIDGDGQVNYE EFVQMMTAK
entity_2: KDARRKQMQLAFAAVNVWRK NLQDR

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts143
1H chemical shifts893

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin, Chain A1
2Calmodulin, Chain B2
3Calcium Ion, 13
4Calcium Ion, 23
5Calcium Ion, 33
6Calcium Ion, 43

Entities:

Entity 1, Calmodulin, Chain A 149 residues - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYTHRILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   THRMETMETALAARGLYSMETLYSASPTHR
9   ASPSERGLUGLUGLUILEARGGLUALAPHE
10   ARGVALPHEASPLYSASPGLYASNGLYTYR
11   ILESERALAALAGLULEUARGHISVALMET
12   THRASNLEUGLYGLULYSLEUTHRASPGLU
13   GLUVALASPGLUMETILEARGGLUALAASP
14   ILEASPGLYASPGLYGLNVALASNTYRGLU
15   GLUPHEVALGLNMETMETTHRALALYS

Entity 2, Calmodulin, Chain B 25 residues - Formula weight is not available

1   LYSASPALAARGARGLYSGLNMETGLNLEU
2   ALAPHEALAALAVALASNVALTRPARGLYS
3   ASNLEUGLNASPARG

Entity 3, Calcium Ion, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, [U-99% 13C; U-99% 15N], 0.4 mM; GluN1 C0 1.0 mM; TRIS, [U-99% 2H], 20 mM; Calcium chloride 1 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 13C-separated NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks