BMRB Entry 51711

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51711

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Title:
1H, 13C, 15N backbone chemical shift assignments for DDX4 1-236
Deposition date:
2022-11-28
Original release date:
2023-11-20
Authors:
Crabtree, Michael; Redfield, Christina; Nott, Tim
Citation:

Citation: Crabtree, Michael; Holland, Jack; Pillai, Arvind; Kompella, Purnima; Babl, Leon; Turner, Noah; Eaton, James; Hochberg, Georg; Aarts, Dirk; Redfield, Christina; Baldwin, Andrew; Nott, Timothy. "Ion binding with charge inversion combined with screening modulates DEAD box helicase phase transitions"  Cell Rep. 42, 113375-113375 (2023).
PubMed: 37980572

Assembly members:

Assembly members:
entity_1, polymer, 236 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pETM-30 containing pGEX-2T-TEV site and pProEx multiple cloning sites

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGDEDWEAEINPHMSSYVPI FEKDRYSGENGDNFNRTPAS SSEMDDGPSRRDHFMKSGFA SGRNFGNRDAGECNKRDNTS TMGGFGVGKSFGNRGFSNSR FEDGDSSGFWRESSNDCEDN PTRNRGFSKRGGYRDGNNSE ASGPYRRGGRGSFRGCRGGF GLGSPNNDLDPDECMQRTGG LFGSRRPVLSGTGNGDTSQS RSGSGSERGGYKGLNEEVIT GSGKNSWKSEAEGGES

Data sets:
Data typeCount
13C chemical shifts511
15N chemical shifts185
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DDX41

Entities:

Entity 1, DDX4 236 residues - Formula weight is not available

1   METGLYASPGLUASPTRPGLUALAGLUILE
2   ASNPROHISMETSERSERTYRVALPROILE
3   PHEGLULYSASPARGTYRSERGLYGLUASN
4   GLYASPASNPHEASNARGTHRPROALASER
5   SERSERGLUMETASPASPGLYPROSERARG
6   ARGASPHISPHEMETLYSSERGLYPHEALA
7   SERGLYARGASNPHEGLYASNARGASPALA
8   GLYGLUCYSASNLYSARGASPASNTHRSER
9   THRMETGLYGLYPHEGLYVALGLYLYSSER
10   PHEGLYASNARGGLYPHESERASNSERARG
11   PHEGLUASPGLYASPSERSERGLYPHETRP
12   ARGGLUSERSERASNASPCYSGLUASPASN
13   PROTHRARGASNARGGLYPHESERLYSARG
14   GLYGLYTYRARGASPGLYASNASNSERGLU
15   ALASERGLYPROTYRARGARGGLYGLYARG
16   GLYSERPHEARGGLYCYSARGGLYGLYPHE
17   GLYLEUGLYSERPROASNASNASPLEUASP
18   PROASPGLUCYSMETGLNARGTHRGLYGLY
19   LEUPHEGLYSERARGARGPROVALLEUSER
20   GLYTHRGLYASNGLYASPTHRSERGLNSER
21   ARGSERGLYSERGLYSERGLUARGGLYGLY
22   TYRLYSGLYLEUASNGLUGLUVALILETHR
23   GLYSERGLYLYSASNSERTRPLYSSERGLU
24   ALAGLUGLYGLYGLUSER

Samples:

sample_1: DDX4, [U-99% 13C; U-99% 15N], 44.6 uM; TCEP 5 mM; PIPES 20 mM; sodium chloride 138.5 mM; D2O, [U-99% 2H], 5%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N BEST-TROSYsample_1isotropicsample_conditions_1
2D 1H-15N BEST-TROSYsample_1isotropicsample_conditions_1
3D BEST-TROSY-HNCAsample_1isotropicsample_conditions_1
3D BEST-TROSY-HNCACBsample_1isotropicsample_conditions_1
3D BEST-TROSY-HN(CO)CACBsample_1isotropicsample_conditions_1
3D BEST-TROSY-HNCOsample_1isotropicsample_conditions_1
3D BEST-TROSY-HN(CA)COsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1
3D HN(COCA)NNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

CcpNMR v2.5 - chemical shift assignment, data analysis

NMRPipe v9.7 - processing

istHMS vV211 - processing

NMR spectrometers:

  • Bruker AVANCE III 750 MHz
  • Bruker AVANCE III 950 MHz

Related Database Links:

UNP DDX4_HUMAN (Q9NQ10)

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks