BMRB Entry 51710

Name: SARS-CoV-2 Phosphorylated Nucleocapsid 1-209 at 35C
Published: 2023-03-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51710

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

SARS-CoV-2 Phosphorylated Nucleocapsid 1-209 at 35C

Deposition date:

2022-11-26

Original release date:

2023-03-07

Authors:

Lee, Eunjeong

Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Saviola, Anthony; Li, Xueni; Ebmeier, Christopher; Kutateladze, Tatiana; Hansen, Kirk Charles; Zhao, Rui; Ahn, Natalie; Sluchanko, Nikolai; Eisenmesser, Elan. "Molecular insight into the specific interactions of the SARS-CoV-2 Nucleocapsid with RNA and host protein" Protein Sci 32, e4603-e4603 (2023).
PubMed: 36807437

Assembly members:

Assembly members:
entity_1, polymer, 209 residues, Formula weight is not available

Natural source:

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPAR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	185
15N chemical shifts	166
1H chemical shifts	166

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SARS-Cov-2 Phosphorylated Nucleocapsid protein	1

Entities:

Entity 1, SARS-Cov-2 Phosphorylated Nucleocapsid protein 209 residues - Formula weight is not available

1

MET

SER

ASP

ASN

GLY

PRO

GLN

ASN

GLN

ARG

2

ASN

ALA

PRO

ARG

ILE

THR

PHE

GLY

PRO

3

SER

ASP

SER

THR

GLY

SER

ASN

GLN

ASN

GLY

4

GLU

ARG

SER

GLY

ALA

ARG

SER

LYS

GLN

ARG

5

ARG

PRO

GLN

GLY

LEU

PRO

ASN

THR

ALA

6

SER

TRP

PHE

THR

ALA

LEU

THR

GLN

HIS

GLY

7

LYS

GLU

ASP

LEU

LYS

PHE

PRO

ARG

GLY

GLN

8

GLY

VAL

PRO

ILE

ASN

THR

ASN

SER

PRO

9

ASP

GLN

ILE

GLY

TYR

ARG

ALA

10

THR

ARG

ILE

ARG

GLY

ASP

GLY

LYS

11

MET

LYS

ASP

LEU

SER

PRO

ARG

TRP

TYR

PHE

12

TYR

LEU

GLY

THR

GLY

PRO

GLU

ALA

GLY

13

LEU

PRO

TYR

GLY

ALA

ASN

LYS

ASP

GLY

ILE

14

ILE

TRP

VAL

ALA

THR

GLU

GLY

ALA

LEU

ASN

15

THR

PRO

LYS

ASP

HIS

ILE

GLY

THR

ARG

ASN

16

PRO

ALA

ASN

ALA

ILE

VAL

LEU

GLN

17

LEU

PRO

GLN

GLY

THR

LEU

PRO

LYS

GLY

18

PHE

TYR

ALA

GLU

GLY

SER

ARG

GLY

SER

19

GLN

ALA

SER

ARG

SER

ARG

SER

20

ARG

ASN

SER

ARG

ASN

SER

THR

PRO

GLY

21

SER

ARG

GLY

THR

SER

PRO

ALA

ARG

Samples:

sample_1: Sars-Cov-2 Nucleocapsid, [U-100% 15N], 500 uM; sodium chloride 150 mM; HEPES 50 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks