BMRB Entry 51703

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51703

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Title:
Recombinant Expression and Chemical Amidation of Isotopically Labeled Native Melittin
Deposition date:
2022-11-23
Original release date:
2023-02-04
Authors:
Gelenter, Martin; Bax, Ad
Citation:

Citation: Gelenter, Martin; Bax, Ad. "Recombinant Expression and Chemical Amidation of Isotopically Labeled Native Melittin"  J. Am. Chem. Soc. 145, 3850-3854 (2023).
PubMed: 36753641

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, 3192.20 Da.

Natural source:

Natural source:   Common Name: honey bee   Taxonomy ID: 7460   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Apis mellifera

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pD454-GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GIGAVLKVLTTGLPALISWI KRKRQQ

Data typeCount
13C chemical shifts94
15N chemical shifts45
1H chemical shifts45
residual dipolar couplings45

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1tetrameric melittin, chain 11
2tetrameric melittin, chain 21
3tetrameric melittin, chain 31
4tetrameric melittin, chain 41

Entities:

Entity 1, tetrameric melittin, chain 1 26 residues - 3192.20 Da.

1   GLYILEGLYALAVALLEULYSVALLEUTHR
2   THRGLYLEUPROALALEUILESERTRPILE
3   LYSARGLYSARGGLNGLN

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