BMRB Entry 51697

Title:
Assignment of pre-miRNA-31 TopA short fragment
Deposition date:
2022-11-18
Original release date:
2023-02-08
Authors:
Kotar, Anita; Keane, Sarah
Citation:

Citation: Ma, Sicong; Kotar, Anita; Grote, Scott; Rouskin, Silvi; Keane, Sarah. "Structure of pre-miR-31 reveals an active role in Dicer processing"  bioRxiv ., .-. (2023).
PubMed: 36711709

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCAUAGCUACGGCUAUGCC

Data sets:
Data typeCount
13C chemical shifts23
1H chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pre-miRNA fragment1

Entities:

Entity 1, pre-miRNA fragment 20 residues - Formula weight is not available

1   GGCAUAGCUA
2   CGGCUAUGCC

Samples:

sample_1: pre-miRNA fragment 0.4 mM; D2O, [U-100% 2H], 100%; potassium phosphate 50 mM; MgCl2 1 mM

sample_conditions_1: ionic strength: 0.0025 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRViewJ v9.2.0-b24 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz