BMRB Entry 51697

Name: Assignment of pre-miRNA-31 TopA short fragment
Published: 2023-02-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51697

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of pre-miRNA-31 TopA short fragment

Deposition date:

2022-11-18

Original release date:

2023-02-08

Authors:

Kotar, Anita; Keane, Sarah

Citation:

Citation: Ma, Sicong; Kotar, Anita; Grote, Scott; Rouskin, Silvi; Keane, Sarah. "Structure of pre-miR-31 reveals an active role in Dicer processing" bioRxiv ., .-. (2023).
PubMed: 36711709

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCAUAGCUACGGCUAUGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	23
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pre-miRNA fragment	1

Entities:

Entity 1, pre-miRNA fragment 20 residues - Formula weight is not available

1		G	G	C	A	U	A	G	C	U	A
2		C	G	G	C	U	A	U	G	C	C

Samples:

sample_1: pre-miRNA fragment 0.4 mM; D2O, [U-100% 2H], 100%; potassium phosphate 50 mM; MgCl2 1 mM

sample_conditions_1: ionic strength: 0.0025 M; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRViewJ v9.2.0-b24 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz