BMRB Entry 51689

Name: Assignments of human CAPRIN1 residues 607-709 N623TN630T mutant
Published: 2022-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51689

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignments of human CAPRIN1 residues 607-709 N623TN630T mutant

Deposition date:

2022-11-08

Original release date:

2022-11-10

Authors:

Toyama, Yuki; Rangadurai, Atul Kaushik; Kay, Lewis

Citation:

Citation: Toyama, Yuki; Rangadurai, Atul Kaushik; Kay, Lewis. "Measurement of 1Halpha transverse relaxation rates in proteins: application to solvent PREs" J. Biomol. NMR 76, 137-152 (2022).
PubMed: 36018482

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petSUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SRGVSRGGSRGARGLMNGYR GPANGFRGGYDGYRPSFSNT PNSGYTQSQFSAPRDYSGYQ RDGYQQNFKRGSGQSGPRGA PRGRGGPPRPNRGMPQMNTQ QVN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	91
15N chemical shifts	91
1H chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Caprin1	1

Entities:

Entity 1, Caprin1 103 residues - Formula weight is not available

1

SER

ARG

GLY

VAL

SER

ARG

GLY

SER

ARG

2

GLY

ALA

ARG

GLY

LEU

MET

THR

GLY

TYR

ARG

3

GLY

PRO

ALA

THR

GLY

PHE

ARG

GLY

TYR

4

ASP

GLY

TYR

ARG

PRO

SER

PHE

SER

ASN

THR

5

PRO

ASN

SER

GLY

TYR

THR

GLN

SER

GLN

PHE

6

SER

ALA

PRO

ARG

ASP

TYR

SER

GLY

TYR

GLN

7

ARG

ASP

GLY

TYR

GLN

ASN

PHE

LYS

ARG

8

GLY

SER

GLY

GLN

SER

GLY

PRO

ARG

GLY

ALA

9

PRO

ARG

GLY

ARG

GLY

PRO

ARG

PRO

10

ASN

ARG

GLY

MET

PRO

GLN

MET

ASN

THR

GLN

11

GLN

VAL

ASN

Samples:

sample_1: Caprin1 residues 607-709 N623TN630T, [U-100% 13C; U-100% 15N], 300 uM; MES 25 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw v10.9 - processing

NMRFAM-SPARKY v1.470 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks