BMRB Entry 51684

Name: Backbone 1H, 13C and 15N Chemical Shift Assignments and Relaxation Parameters for USP14-Ubl domain
Published: 2024-04-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51684

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Chemical Shift Assignments and Relaxation Parameters for USP14-Ubl domain

Deposition date:

2022-11-02

Original release date:

2024-04-15

Authors:

Salomonsson, Johannes; Wallner, Bjorn; Sjostrand, Linda; D'Arcy, Padraig; Ahlner, Alexandra; Sunnerhagen, Maria

Citation:

Citation: Salomonsson, Johannes; Wallner, Bjorn; Sjostrand, Linda; D'Arcy, Padraig; Sunnerhagen, Maria; Ahlner, Alexandra. "Transient interdomain interactions in free USP14 shape its conformational ensemble" Protein Sci. 33, e4975-e4975 (2024).
PubMed: 38588275

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pNIC-28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPLYSVTVKWGKEKFEGVEL NTDEPPMVFKAQLFALTGVQ PARQKVMVKGGTLKDDDWGN IKIKNGMTLLMMGSADALPE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
heteronucl_NOEs
- heteronucl_NOEs_1
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1
order_parameters
- order_parameters_1

Data type	Count
13C chemical shifts	226
15N chemical shifts	73
1H chemical shifts	73
T1 relaxation values	69
T2 relaxation values	69
heteronuclear NOE values	69
order parameters	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubl domain	1

Entities:

Entity 1, Ubl domain 80 residues - Formula weight is not available

1	MET	PRO	LEU	TYR	SER	VAL	THR	VAL	LYS	TRP
2	GLY	LYS	GLU	LYS	PHE	GLU	GLY	VAL	GLU	LEU
3	ASN	THR	ASP	GLU	PRO	PRO	MET	VAL	PHE	LYS
4	ALA	GLN	LEU	PHE	ALA	LEU	THR	GLY	VAL	GLN
5	PRO	ALA	ARG	GLN	LYS	VAL	MET	VAL	LYS	GLY
6	GLY	THR	LEU	LYS	ASP	ASP	ASP	TRP	GLY	ASN
7	ILE	LYS	ILE	LYS	ASN	GLY	MET	THR	LEU	LEU
8	MET	MET	GLY	SER	ALA	ASP	ALA	LEU	PRO	GLU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
T1/R1 relaxation	sample_1	isotropic	sample_conditions_1
T1rho/R1rho relaxation	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 51684

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	PRO	LEU	TYR	SER	VAL	THR	VAL	LYS	TRP
2	GLY	LYS	GLU	LYS	PHE	GLU	GLY	VAL	GLU	LEU
3	ASN	THR	ASP	GLU	PRO	PRO	MET	VAL	PHE	LYS
4	ALA	GLN	LEU	PHE	ALA	LEU	THR	GLY	VAL	GLN
5	PRO	ALA	ARG	GLN	LYS	VAL	MET	VAL	LYS	GLY
6	GLY	THR	LEU	LYS	ASP	ASP	ASP	TRP	GLY	ASN
7	ILE	LYS	ILE	LYS	ASN	GLY	MET	THR	LEU	LEU
8	MET	MET	GLY	SER	ALA	ASP	ALA	LEU	PRO	GLU

1	MET	PRO	LEU	TYR	SER	VAL	THR	VAL	LYS	TRP
2	GLY	LYS	GLU	LYS	PHE	GLU	GLY	VAL	GLU	LEU
3	ASN	THR	ASP	GLU	PRO	PRO	MET	VAL	PHE	LYS
4	ALA	GLN	LEU	PHE	ALA	LEU	THR	GLY	VAL	GLN
5	PRO	ALA	ARG	GLN	LYS	VAL	MET	VAL	LYS	GLY
6	GLY	THR	LEU	LYS	ASP	ASP	ASP	TRP	GLY	ASN
7	ILE	LYS	ILE	LYS	ASN	GLY	MET	THR	LEU	LEU
8	MET	MET	GLY	SER	ALA	ASP	ALA	LEU	PRO	GLU

1	MET	PRO	LEU	TYR	SER	VAL	THR	VAL	LYS	TRP
2	GLY	LYS	GLU	LYS	PHE	GLU	GLY	VAL	GLU	LEU
3	ASN	THR	ASP	GLU	PRO	PRO	MET	VAL	PHE	LYS
4	ALA	GLN	LEU	PHE	ALA	LEU	THR	GLY	VAL	GLN
5	PRO	ALA	ARG	GLN	LYS	VAL	MET	VAL	LYS	GLY
6	GLY	THR	LEU	LYS	ASP	ASP	ASP	TRP	GLY	ASN
7	ILE	LYS	ILE	LYS	ASN	GLY	MET	THR	LEU	LEU
8	MET	MET	GLY	SER	ALA	ASP	ALA	LEU	PRO	GLU