BMRB Entry 51684

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51684

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments and Relaxation Parameters for USP14-Ubl domain
Deposition date:
2022-11-02
Original release date:
2024-04-15
Authors:
Salomonsson, Johannes; Wallner, Bjorn; Sjostrand, Linda; D'Arcy, Padraig; Ahlner, Alexandra; Sunnerhagen, Maria
Citation:

Citation: Salomonsson, Johannes; Wallner, Bjorn; Sjostrand, Linda; D'Arcy, Padraig; Sunnerhagen, Maria; Ahlner, Alexandra. "Transient interdomain interactions in free USP14 shape its conformational ensemble"  Protein Sci. 33, e4975-e4975 (2024).
PubMed: 38588275

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC-28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPLYSVTVKWGKEKFEGVEL NTDEPPMVFKAQLFALTGVQ PARQKVMVKGGTLKDDDWGN IKIKNGMTLLMMGSADALPE

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts73
1H chemical shifts73
T1 relaxation values69
T2 relaxation values69
heteronuclear NOE values69
order parameters69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubl domain1

Entities:

Entity 1, Ubl domain 80 residues - Formula weight is not available

1   METPROLEUTYRSERVALTHRVALLYSTRP
2   GLYLYSGLULYSPHEGLUGLYVALGLULEU
3   ASNTHRASPGLUPROPROMETVALPHELYS
4   ALAGLNLEUPHEALALEUTHRGLYVALGLN
5   PROALAARGGLNLYSVALMETVALLYSGLY
6   GLYTHRLEULYSASPASPASPTRPGLYASN
7   ILELYSILELYSASNGLYMETTHRLEULEU
8   METMETGLYSERALAASPALALEUPROGLU

Samples:

sample_1: USP14-Ubl, [U-99% 13C; U-99% 15N], 0.4 mM; HEPES 20 mM; sodium chloride 100 mM; TCEP 0.5 mM; sodium azide 0.02 mM

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
T1/R1 relaxationsample_1isotropicsample_conditions_1
T1rho/R1rho relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

PINT - data analysis

COMPASS - chemical shift assignment

NMRFAM-SPARKY - chemical shift assignment, peak picking

Tensor2 - data analysis

qMDD - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks