BMRB Entry 51682

Name: Chemical_shift_FAM122A_29-120
Published: 2023-10-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51682

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical_shift_FAM122A_29-120

Deposition date:

2022-11-01

Original release date:

2023-10-19

Authors:

Vos, Margaret; Page, Rebecca; Peti, Wolfgang

Citation:

Citation: Padi, Sathish; Vos, Margaret; Godek, Rachel; Fuller, James; Kruse, Thomas; Hein, Jamin; Nilsson, Jakob; Kelker, Matthew; Page, Rebecca; Peti, Wolfgang. "Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19" Nature 625, 195-203 (2024).
PubMed: 38123684

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, 10405 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HMGGGLRRSNSAPLIHGLSD SSPVFQAEAPSARRNSTTFP SRHGLLLPASPVRMHSSRLH QIKQEEGMDLINRETVHERE VQTAMQISHSWEES

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	243
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAM122A_29-120	1

Entities:

Entity 1, FAM122A_29-120 94 residues - 10405 Da.

FAM122A residues 29-120; 92 residues starting from with GGG

1

HIS

MET

GLY

LEU

ARG

SER

ASN

2

SER

ALA

PRO

LEU

ILE

HIS

GLY

LEU

SER

ASP

3

SER

PRO

VAL

PHE

GLN

ALA

GLU

ALA

PRO

4

SER

ALA

ARG

ASN

SER

THR

PHE

PRO

5

SER

ARG

HIS

GLY

LEU

PRO

ALA

SER

6

PRO

VAL

ARG

MET

HIS

SER

ARG

LEU

HIS

7

GLN

ILE

LYS

GLN

GLU

GLY

MET

ASP

LEU

8

ILE

ASN

ARG

GLU

THR

VAL

HIS

GLU

ARG

GLU

9

VAL

GLN

THR

ALA

MET

GLN

ILE

SER

HIS

SER

10

TRP

GLU

SER

Samples:

sample_1: FAM122A_29-120, [U-99% 15N], 400 uM; FAM122A_29-120, [U-99% 13C; U-99% 15N], 400 uM; Na2HPO4 20 mM; NaCl 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.1 - collection, processing

CARA vCARA_1.9 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks