BMRB Entry 51678

Title:
13C and 15N Resonance Assignments of Alpha Synuclein Fibrils Amplified from Dementia with Lewy Bodies Tissue
Deposition date:
2022-10-28
Original release date:
2023-03-14
Authors:
Barclay, Alexander; Dhavale, Dhruva; Borcik, Collin; Milchberg, Moses; Kotzbauer, Paul; Rienstra, Chad
Citation:

Citation: Barclay, Alexander; Dhavale, Dhruva; Borcik, Collin; Milchberg, Moses; Kotzbauer, Paul; Rienstra, Chad. "13C and 15N resonance assignments of alpha synuclein fibrils amplified from Lewy Body Dementia tissue"  Biomol. NMR Assign. 17, 281-286 (2023).
PubMed: 37919529

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a-AS

Data sets:
Data typeCount
13C chemical shifts190
15N chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein1

Entities:

Entity 1, alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: alpha-synuclein, [U-100% 13C; U-100% 15N], 4.5 mg

sample_conditions_1: pH: 7.6; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_1
3D NCACX DARRsample_1isotropicsample_conditions_1
3D NCOCX DARRsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
2D 13C-13C DARRsample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D NCOsample_1isotropicsample_conditions_1
3D NCOCA SPC5sample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
2D 13C-13C SPC7sample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Agilent VNMRS 500 MHz
  • Agilent VNMRS 750 MHz