BMRB Entry 51677

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51677

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Title:
DinB
Deposition date:
2022-10-28
Original release date:
2023-06-23
Authors:
Burmann, Bjorn; Okeke, Damasus
Citation:

Citation: Okeke, Damasus; Lidman, Jens; Matecko-Burmann, Irena; Burmann, Bjorn. "Thumb-domain dynamics modulate the functional repertoire of DNA-Polymerase IV (DinB)"  Nucleic Acids Res. 51, 7036-7052 (2023).
PubMed: 37260088

Assembly members:

Assembly members:
entity_1, polymer, 371 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MRKIIHVDMDCFFAAVEMRD NPALRDIPIAIGGSRERRGV ISTANYPARKFGVRSAMPTG MALKLCPHLTLLPGRFDAYK EASNHIREIFSRYTSRIEPL SLDEAYLDVTDSVHCHGSAT LIAQEIRQTIFNELQLTASA GVAPVKFLAKIASDMNKPNG QFVITPAEVPAFLQTLPLAK IPGVGKVSAAKLEAMGLRTC GDVQKCDLVMLLKRFGKFGR ILWERSQGIDERDVNSERLR KSVGVERTMAEDIHHWSECE AIIERLYPELERRLAKVKPD LLIARQGVKLKFDDFQQTTQ EHVWPRLNKADLIATARKTW DERRGGRGVRLVGLHVTLLD PQMERQLVLGL

Data sets:
Data typeCount
13C chemical shifts914
15N chemical shifts309
1H chemical shifts660

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DinB1

Entities:

Entity 1, DinB 371 residues - Formula weight is not available

Including N-terminal hexa-histidin tag

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METARGLYSILEILEHISVALASPMETASP
4   CYSPHEPHEALAALAVALGLUMETARGASP
5   ASNPROALALEUARGASPILEPROILEALA
6   ILEGLYGLYSERARGGLUARGARGGLYVAL
7   ILESERTHRALAASNTYRPROALAARGLYS
8   PHEGLYVALARGSERALAMETPROTHRGLY
9   METALALEULYSLEUCYSPROHISLEUTHR
10   LEULEUPROGLYARGPHEASPALATYRLYS
11   GLUALASERASNHISILEARGGLUILEPHE
12   SERARGTYRTHRSERARGILEGLUPROLEU
13   SERLEUASPGLUALATYRLEUASPVALTHR
14   ASPSERVALHISCYSHISGLYSERALATHR
15   LEUILEALAGLNGLUILEARGGLNTHRILE
16   PHEASNGLULEUGLNLEUTHRALASERALA
17   GLYVALALAPROVALLYSPHELEUALALYS
18   ILEALASERASPMETASNLYSPROASNGLY
19   GLNPHEVALILETHRPROALAGLUVALPRO
20   ALAPHELEUGLNTHRLEUPROLEUALALYS
21   ILEPROGLYVALGLYLYSVALSERALAALA
22   LYSLEUGLUALAMETGLYLEUARGTHRCYS
23   GLYASPVALGLNLYSCYSASPLEUVALMET
24   LEULEULYSARGPHEGLYLYSPHEGLYARG
25   ILELEUTRPGLUARGSERGLNGLYILEASP
26   GLUARGASPVALASNSERGLUARGLEUARG
27   LYSSERVALGLYVALGLUARGTHRMETALA
28   GLUASPILEHISHISTRPSERGLUCYSGLU
29   ALAILEILEGLUARGLEUTYRPROGLULEU
30   GLUARGARGLEUALALYSVALLYSPROASP
31   LEULEUILEALAARGGLNGLYVALLYSLEU
32   LYSPHEASPASPPHEGLNGLNTHRTHRGLN
33   GLUHISVALTRPPROARGLEUASNLYSALA
34   ASPLEUILEALATHRALAARGLYSTHRTRP
35   ASPGLUARGARGGLYGLYARGGLYVALARG
36   LEUVALGLYLEUHISVALTHRLEULEUASP
37   PROGLNMETGLUARGGLNLEUVALLEUGLY
38   LEU

Samples:

sample_1: DinB, [U-100% 13C; U-100% 15N; U-100% 2H], 400 uM; KPi 50 mM; KCl 300 mM; Arginine 50 mM; Glutamine 50 mM; EDTA 1 mM; TCEP 1 mM; CHAPSO 0.1 mM; D2O 10%

sample_2: DinB, [U-12C; U-15N; U-2H; 99% 1HD-Ile; 99% 2HD-Leu; 99% 2HG-Val; 99% HE-Met; 99% HB-Ala]], 400 uM; KPi 50 mM; KCl 300 mM; Arginine 50 mM; Glutamine 50 mM; EDTA 1 mM; TCEP 1 mM; CHAPSO 0.1 mM; D2O 10%

sample_conditions_1: ionic strength: 400 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
HNCAsample_1isotropicsample_conditions_1
HNCACBsample_1isotropicsample_conditions_1
HN(CO)CACBsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
3D 13C-separated NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks