BMRB Entry 51656

Name: The 1H, 15N chemical shifts of carboxyl terminus of A Family A G protein coupled receptor with Gs
Published: 2023-06-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51656

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The 1H, 15N chemical shifts of carboxyl terminus of A Family A G protein coupled receptor with Gs

Deposition date:

2022-10-06

Original release date:

2023-06-23

Authors:

Heng, Jie; Yang, Yujie; Niu, Xiaogang; Jin, Changwen

Citation:

Citation: Heng, Jie; Hu, Yunfei; Perez-Hernandez, Guillermo; Inoue, Asuka; Zhao, Jiawei; Ma, Xiuyan; Sun, Xiaoou; Kawakami, Kouki; Ikuta, Tatsuya; Ding, Jienv; Yang, Yujie; Zhang, Lujia; Peng, Sijia; Niu, Xiaogang; Li, Hongwei; Guixa-Gonzalez, Ramon; Jin, Changwen; Hildebrand, Peter; Chen, Chunlai; Kobilka, Brian. "Function and dynamics of the intrinsically disordered carboxyl terminus of b2 adrenergic receptor" Nat. Commun. 14, 2005-2005 (2023).
PubMed: 37037825

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available
entity_2, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX 6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: LKAYGNGYSSNGNTGEQSGY HVEQEKENKLLAEDLPGTED FVGHQGTVPSDNIDSQGRNA STNDSLL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	50
1H chemical shifts	50

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	B2AR_Cter	1
2	Gs	2

Entities:

Entity 1, B2AR_Cter 67 residues - Formula weight is not available

1

LEU

LYS

ALA

TYR

GLY

ASN

GLY

TYR

SER

2

ASN

GLY

ASN

THR

GLY

GLU

GLN

SER

GLY

TYR

3

HIS

VAL

GLU

GLN

GLU

LYS

GLU

ASN

LYS

LEU

4

LEU

ALA

GLU

ASP

LEU

PRO

GLY

THR

GLU

ASP

5

PHE

VAL

GLY

HIS

GLN

GLY

THR

VAL

PRO

SER

6

ASP

ASN

ILE

ASP

SER

GLN

GLY

ARG

ASN

ALA

7

SER

THR

ASN

ASP

SER

LEU

Entity 2, Gs - Formula weight is not available

Samples:

sample_1: B2AR_Cter, [U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks