BMRB Entry 51654

Title:
Backbone N and HN Chemical Shift Assignments for RBPMS-A (1-122)
Deposition date:
2022-10-06
Original release date:
2022-10-10
Authors:
Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher
Citation:

Citation: Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher. "Phosphorylation of the smooth muscle master splicing regulator RBPMS regulates its splicing activity"  Nucleic Acids Res. 50, 11895-11915 (2022).
PubMed: 36408906

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBPMS-A1

Entities:

Entity 1, RBPMS-A 122 residues - Formula weight is not available

1   METASNGLYGLYGLYLYSALAGLULYSGLU
2   ASNTHRPROSERGLUALAASNLEUGLNGLU
3   GLUGLUVALARGTHRLEUPHEVALSERGLY
4   LEUPROLEUASPILELYSPROARGGLULEU
5   TYRLEULEUPHEARGPROPHELYSGLYTYR
6   GLUGLYSERLEUILELYSLEUTHRSERLYS
7   GLNPROVALGLYPHEVALSERPHEASPSER
8   ARGSERGLUALAGLUALAALALYSASNALA
9   LEUASNGLYILEARGPHEASPPROGLUILE
10   PROGLNTHRLEUARGLEUGLUPHEALALYS
11   ALAASNTHRLYSMETALALYSASNLYSLEU
12   VALGLYTHRPROASNPROSERTHRPROLEU
13   PROASN

Samples:

sample_1: RBPMS-A, [U-98% 13C; U-98% 15N], 0.5 mM; HEPES 10 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks