BMRB Entry 51654

Name: Backbone N and HN Chemical Shift Assignments for RBPMS-A (1-122)
Published: 2022-10-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51654

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone N and HN Chemical Shift Assignments for RBPMS-A (1-122)

Deposition date:

2022-10-06

Original release date:

2022-10-10

Authors:

Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher

Citation:

Citation: Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher. "Phosphorylation of the smooth muscle master splicing regulator RBPMS regulates its splicing activity" Nucleic Acids Res. 50, 11895-11915 (2022).
PubMed: 36408906

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MNGGGKAEKENTPSEANLQE EEVRTLFVSGLPLDIKPREL YLLFRPFKGYEGSLIKLTSK QPVGFVSFDSRSEAEAAKNA LNGIRFDPEIPQTLRLEFAK ANTKMAKNKLVGTPNPSTPL PN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	94
1H chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RBPMS-A	1

Entities:

Entity 1, RBPMS-A 122 residues - Formula weight is not available

1

MET

ASN

GLY

LYS

ALA

GLU

LYS

GLU

2

ASN

THR

PRO

SER

GLU

ALA

ASN

LEU

GLN

GLU

3

GLU

VAL

ARG

THR

LEU

PHE

VAL

SER

GLY

4

LEU

PRO

LEU

ASP

ILE

LYS

PRO

ARG

GLU

LEU

5

TYR

LEU

PHE

ARG

PRO

PHE

LYS

GLY

TYR

6

GLU

GLY

SER

LEU

ILE

LYS

LEU

THR

SER

LYS

7

GLN

PRO

VAL

GLY

PHE

VAL

SER

PHE

ASP

SER

8

ARG

SER

GLU

ALA

GLU

ALA

LYS

ASN

ALA

9

LEU

ASN

GLY

ILE

ARG

PHE

ASP

PRO

GLU

ILE

10

PRO

GLN

THR

LEU

ARG

LEU

GLU

PHE

ALA

LYS

11

ALA

ASN

THR

LYS

MET

ALA

LYS

ASN

LYS

LEU

12

VAL

GLY

THR

PRO

ASN

PRO

SER

THR

PRO

LEU

13

PRO

ASN

Samples:

sample_1: RBPMS-A, [U-98% 13C; U-98% 15N], 0.5 mM; HEPES 10 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 6.8; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks