BMRB Entry 51653

Title:
the carboxyl terminus of A Family A G protein coupled receptor
Deposition date:
2022-10-05
Original release date:
2023-06-23
Authors:
Heng, Jie; Yang, Yujie; Niu, Xiaogang; Jin, Changwen
Citation:

Citation: Heng, Jie; Hu, Yunfei; Perez-Hernandez, Guillermo; Inoue, Asuka; Zhao, Jiawei; Ma, Xiuyan; Sun, Xiaoou; Kawakami, Kouki; Ikuta, Tatsuya; Ding, Jienv; Yang, Yujie; Zhang, Lujia; Peng, Sijia; Niu, Xiaogang; Li, Hongwei; Guixa-Gonzalez, Ramon; Jin, Changwen; Hildebrand, Peter; Chen, Chunlai; Kobilka, Brian. "Function and dynamics of the intrinsically disordered carboxyl terminus of b2 adrenergic receptor"  Nat. Commun. 14, 2005-2005 (2023).
PubMed: 37037825

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 6p-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts50
1H chemical shifts50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1b2AR C-ter1

Entities:

Entity 1, b2AR C-ter 67 residues - Formula weight is not available

1   LEULYSALATYRGLYASNGLYTYRSERSER
2   ASNGLYASNTHRGLYGLUGLNSERGLYTYR
3   HISVALGLUGLNGLULYSGLUASNLYSLEU
4   LEUALAGLUASPLEUPROGLYTHRGLUASP
5   PHEVALGLYHISGLNGLYTHRVALPROSER
6   ASPASNILEASPSERGLNGLYARGASNALA
7   SERTHRASNASPSERLEULEU

Samples:

sample_1: b2AR C-ter, [U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks