BMRB Entry 51651

Name: Rev7/Rev3-RBM1
Published: 2023-06-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51651

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rev7/Rev3-RBM1

Deposition date:

2022-10-04

Original release date:

2023-06-23

Authors:

Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry

Citation:

Citation: Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry. "Backbone and ILV side-chain methyl NMR resonance assignments of human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes" Biomol. NMR Assign. 17, 107-114 (2023).
PubMed: 37129702

Assembly members:

Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available
entity_2, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-Duet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTTLTRQDLNFGQVVADVLC EFLEVAVHLILYVREVYPVG IFQKRKKYNVPVQMSCHPEL NQYIQDTLHCVKPLLEKNDV EKVVVVILDKEHRPVEKFVF EITQPPLLSISSDSLLSHVE QLLAAFILKISVCDAVLDHN PPGCTFTVLVHTREAATRNM EKIQVIKDFPWILADEQDVH MHDPRLIPLKTMTSDILKMQ LYVEERAHKGS
entity_2: MRTANILKPLMSPPSREEIM ATLLDHD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	772
15N chemical shifts	208
1H chemical shifts	484

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rev7	1
2	Rev3-RBM1	2

Entities:

Entity 1, Rev7 211 residues - Formula weight is not available

1

MET

THR

LEU

THR

ARG

GLN

ASP

LEU

ASN

2

PHE

GLY

GLN

VAL

ALA

ASP

VAL

LEU

CYS

3

GLU

PHE

LEU

GLU

VAL

ALA

VAL

HIS

LEU

ILE

4

LEU

TYR

VAL

ARG

GLU

VAL

TYR

PRO

VAL

GLY

5

ILE

PHE

GLN

LYS

ARG

LYS

TYR

ASN

VAL

6

PRO

VAL

GLN

MET

SER

CYS

HIS

PRO

GLU

LEU

7

ASN

GLN

TYR

ILE

GLN

ASP

THR

LEU

HIS

CYS

8

VAL

LYS

PRO

LEU

GLU

LYS

ASN

ASP

VAL

9

GLU

LYS

VAL

ILE

LEU

ASP

LYS

10

GLU

HIS

ARG

PRO

VAL

GLU

LYS

PHE

VAL

PHE

11

GLU

ILE

THR

GLN

PRO

LEU

SER

ILE

12

SER

ASP

SER

LEU

SER

HIS

VAL

GLU

13

GLN

LEU

ALA

PHE

ILE

LEU

LYS

ILE

14

SER

VAL

CYS

ASP

ALA

VAL

LEU

ASP

HIS

ASN

15

PRO

GLY

CYS

THR

PHE

THR

VAL

LEU

VAL

16

HIS

THR

ARG

GLU

ALA

THR

ARG

ASN

MET

17

GLU

LYS

ILE

GLN

VAL

ILE

LYS

ASP

PHE

PRO

18

TRP

ILE

LEU

ALA

ASP

GLU

GLN

ASP

VAL

HIS

19

MET

HIS

ASP

PRO

ARG

LEU

ILE

PRO

LEU

LYS

20

THR

MET

THR

SER

ASP

ILE

LEU

LYS

MET

GLN

21

LEU

TYR

VAL

GLU

ARG

ALA

HIS

LYS

GLY

22

SER

Entity 2, Rev3-RBM1 27 residues - Formula weight is not available

1

MET

ARG

THR

ALA

ASN

ILE

LEU

LYS

PRO

LEU

2

MET

SER

PRO

SER

ARG

GLU

ILE

MET

3

ALA

THR

LEU

ASP

HIS

ASP

Samples:

sample_1: Rev7R124A, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM; Rev3-RBM1, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM

sample_2: Rev7K44A,R124A,A135D, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM; Rev3-RBM1, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HMCMCGCBCA	sample_1	isotropic	sample_conditions_1
4D 13C-1H HMQC-NOESY-TROSY	sample_2	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
13C-13C HMQC-NOESY-HMQC	sample_2	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

SPARTA+ - chemical shift calculation

NMRPipe - processing

TALOS-N - structure solution

NMR spectrometers:

Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks