BMRB Entry 51650

Title:
Ded1p N-terminal IDR
Deposition date:
2022-10-04
Original release date:
2022-10-10
Authors:
Schneider, Jakob
Citation:

Citation: schneider, Jakob. "Ded1p N-terminal IDR"  to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ded1p N-terminal IDR1

Entities:

Entity 1, Ded1p N-terminal IDR 81 residues - Formula weight is not available

1   METALAGLULEUSERGLUGLNVALGLNASN
2   LEUSERILEASNASPASNASNGLUASNGLY
3   TYRVALPROPROHISLEUARGGLYLYSPRO
4   ARGSERALAARGASNASNSERSERASNTYR
5   ASNASNASNASNGLYGLYTYRASNGLYGLY
6   ARGGLYGLYGLYSERPHEPHESERASNASN
7   ARGARGGLYGLYTYRGLYASNGLYGLYPHE
8   PHEGLYGLYASNASNGLYGLYSERARGSER
9   ASN

Samples:

sample_1: Ded1p N-terminal IDR, [U-13C; U-15N], 300 uM; sodium chloride 125 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks