BMRB Entry 51649

Name: Ded1p RecA2 domain
Published: 2022-10-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51649

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Ded1p RecA2 domain

Deposition date:

2022-10-04

Original release date:

2022-10-10

Authors:

Hubner, Julian

Citation:

Citation: Hubner, Julian. "Ded1p RecA2 domain" to be published ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: STSENITQKVLYVENQDKKS ALLDLLSASTDGLTLIFVET KRMADQLTDFLIMQNFRATA IHGDRTQSERERALAAFRSG AATLLVATAVAARGLDIPNV THVINYDLPSDVDDYVHRIG RTGRAGNTGLATAFFNSENS NIVKGLHEILTEANQEVPSF LKDAMMS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	562
15N chemical shifts	162
1H chemical shifts	474

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ded1p RecA2	1

Entities:

Entity 1, Ded1p RecA2 167 residues - Formula weight is not available

1

SER

THR

SER

GLU

ASN

ILE

THR

GLN

LYS

VAL

2

LEU

TYR

VAL

GLU

ASN

GLN

ASP

LYS

SER

3

ALA

LEU

ASP

LEU

SER

ALA

SER

THR

4

ASP

GLY

LEU

THR

LEU

ILE

PHE

VAL

GLU

THR

5

LYS

ARG

MET

ALA

ASP

GLN

LEU

THR

ASP

PHE

6

LEU

ILE

MET

GLN

ASN

PHE

ARG

ALA

THR

ALA

7

ILE

HIS

GLY

ASP

ARG

THR

GLN

SER

GLU

ARG

8

GLU

ARG

ALA

LEU

ALA

PHE

ARG

SER

GLY

9

ALA

THR

LEU

VAL

ALA

THR

ALA

VAL

10

ALA

ARG

GLY

LEU

ASP

ILE

PRO

ASN

VAL

11

THR

HIS

VAL

ILE

ASN

TYR

ASP

LEU

PRO

SER

12

ASP

VAL

ASP

TYR

VAL

HIS

ARG

ILE

GLY

13

ARG

THR

GLY

ARG

ALA

GLY

ASN

THR

GLY

LEU

14

ALA

THR

ALA

PHE

ASN

SER

GLU

ASN

SER

15

ASN

ILE

VAL

LYS

GLY

LEU

HIS

GLU

ILE

LEU

16

THR

GLU

ALA

ASN

GLN

GLU

VAL

PRO

SER

PHE

17

LEU

LYS

ASP

ALA

MET

SER

Samples:

sample_1: Ded1p RecA2, [U-13C; U-15N], 2 mM; sodium chloride 125 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks