BMRB Entry 51645

Title:
Full-length NaK ILV chemical shifts
Deposition date:
2022-09-29
Original release date:
2022-11-21
Authors:
Henzler-Wildman, Katherine
Citation:

Citation: Kurauskas, Vilius; Tonelli, Marco; Henzler-Wildman, Katherine. "Full opening of helix bundle does not lead to NaK channel activation"  J. Gen. Physiol. 154, e202213196-e202213196 (2022).
PubMed: 36326620

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus cereus   Taxonomy ID: 1396   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts51
15N chemical shifts21
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NaK1

Entities:

Entity 1, NaK 118 residues - Formula weight is not available

Residues -3 to 1 - residual after cleavage with thrombin

1   GLYSERHISMETALALEUSERPHELEULEU
2   THRLEULYSARGMETLEUARGALACYSLEU
3   ARGALATRPLYSASPLYSGLUPHEGLNVAL
4   LEUPHEVALLEUTHRILELEUTHRLEUILE
5   SERGLYTHRILEPHETYRSERTHRVALGLU
6   GLYLEUARGPROILEASPALALEUTYRPHE
7   SERVALVALTHRLEUTHRTHRVALGLYASP
8   GLYASNPHESERPROGLNTHRASPPHEGLY
9   LYSILEPHETHRILELEUTYRILEPHEILE
10   GLYILEGLYLEUVALPHEGLYPHEILEHIS
11   LYSLEUALAVALASNVALGLNLEUPROSER
12   ILELEUSERASNARGLYSLYSGLU

Samples:

sample_1: Full-length NaK, [U-15N; U-2H, ILV 13C, 1H], 0.76 mM; MOPS 100 mM; KCl 100 mM; DMPC, [U-99% 2H], 44 mM; DHPC, [U-99% 2H], 113 mM; DSS 50 uM; NaN3 0.1 mg

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D 13C, 13C NOESY HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe vVersion 9.0 - data analysis

TOPSPIN v3.6.2 - collection

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Related Database Links:

UNP Q81HW2
AlphaFold Q81HW2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks