BMRB Entry 51638

Name: Backbone assignment of cJun TAD 1-151
Published: 2022-09-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51638

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of cJun TAD 1-151

Deposition date:

2022-09-22

Original release date:

2022-09-26

Authors:

Waudby, Christopher; Alvarez-Teijeiro, Saul; Ruiz, E.; Suppinger, Simon; Pinotsis, Nikos; Brown, Paul; Behrens, Axel; Christodoulou, John; Mylona, Anastasia

Citation:

Citation: Waudby, Christopher; Alvarez-Teijeiro, Saul; Ruiz, E.; Suppinger, Simon; Pinotsis, Nikos; Brown, Paul; Behrens, Axel; Christodoulou, John; Mylona, Anastasia. "An intrinsic temporal order of c-JUN N-terminal phosphorylation regulates its activity by orchestrating co-factor recruitment" Nat. Commun. 13, 6133-6133 (2022).
PubMed: 36253406

Assembly members:

Assembly members:
entity_1, polymer, 154 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGSTAKMETTFYDDALNAS FLPSESGPYGYSNPKILKQS MTLNLADPVGSLKPHLRAKN SDLLTSPDVGLLKLASPELE RLIIQSSNGHITTTPTPTQF LCPKNVTDEQEGFAEGFVRA LAELHSQNTLPSVTSAAQPV NGAGMVAPAVASVA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	438
15N chemical shifts	135
1H chemical shifts	135

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cJun	1

Entities:

Entity 1, cJun 154 residues - Formula weight is not available

Residues 1-4 represent a TEV cleavage site

1

GLY

PRO

GLY

SER

THR

ALA

LYS

MET

GLU

THR

2

THR

PHE

TYR

ASP

ALA

LEU

ASN

ALA

SER

3

PHE

LEU

PRO

SER

GLU

SER

GLY

PRO

TYR

GLY

4

TYR

SER

ASN

PRO

LYS

ILE

LEU

LYS

GLN

SER

5

MET

THR

LEU

ASN

LEU

ALA

ASP

PRO

VAL

GLY

6

SER

LEU

LYS

PRO

HIS

LEU

ARG

ALA

LYS

ASN

7

SER

ASP

LEU

THR

SER

PRO

ASP

VAL

GLY

8

LEU

LYS

LEU

ALA

SER

PRO

GLU

LEU

GLU

9

ARG

LEU

ILE

GLN

SER

ASN

GLY

HIS

10

ILE

THR

PRO

THR

PRO

THR

GLN

PHE

11

LEU

CYS

PRO

LYS

ASN

VAL

THR

ASP

GLU

GLN

12

GLU

GLY

PHE

ALA

GLU

GLY

PHE

VAL

ARG

ALA

13

LEU

ALA

GLU

LEU

HIS

SER

GLN

ASN

THR

LEU

14

PRO

SER

VAL

THR

SER

ALA

GLN

PRO

VAL

15

ASN

GLY

ALA

GLY

MET

VAL

ALA

PRO

ALA

VAL

16

ALA

SER

VAL

ALA

Samples:

sample_1: cJun TAD, [U-100% 13C; U-100% 15N], 150 uM; K2HPO4 10 mM; NaCl 150 mM; MgCl2 5 mM; DSS 0.01 w/v

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4 - chemical shift assignment, peak picking

TOPSPIN v3.5pl6 - collection

NMRPipe v10.9 - processing

NMR spectrometers:

Bruker AVANCE III 700 MHz

UNP	P05412
AlphaFold	Q96G93