BMRB Entry 51637
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51637
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NMR-STAR v3 text file.
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Citation: Berardi, Andrea; Botrugno, Oronza; Quilici, Giacomo; Garcia Manteiga, Jose Manuel; Bachi, Angela; Tonon, Giovanni; Musco, Giovanna. "Nizp1 is a specific NUP98-NSD1 functional interactor that regulates NUP98-NSD1-dependent oncogenic programs" FEBS J. 290, 1782-1797 (2022).
PubMed: 36271682
Assembly members:
entity_1, polymer, 105 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11SUMO3
Entity Sequences (FASTA):
entity_1: SRRAKGEGKRQSEDECFRCG
DGGQLVLCDRKFCTKAYHLS
CLGLGKRPFGKWECPWHHCD
VCGKPSTSFCHLCPNSFCKE
HQDGTAFSCTPDGRSYCSEH
DLGAA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NSD2_PHDvC5HCH | 1 |
| 2 | Zn_1 | 2 |
| 3 | Zn_2 | 2 |
| 4 | Zn_3 | 2 |
| 5 | Zn_4 | 2 |
Entities:
Entity 1, NSD2_PHDvC5HCH 105 residues - Formula weight is not available
| 1 | SER | ARG | ARG | ALA | LYS | GLY | GLU | GLY | LYS | ARG | ||||
| 2 | GLN | SER | GLU | ASP | GLU | CYS | PHE | ARG | CYS | GLY | ||||
| 3 | ASP | GLY | GLY | GLN | LEU | VAL | LEU | CYS | ASP | ARG | ||||
| 4 | LYS | PHE | CYS | THR | LYS | ALA | TYR | HIS | LEU | SER | ||||
| 5 | CYS | LEU | GLY | LEU | GLY | LYS | ARG | PRO | PHE | GLY | ||||
| 6 | LYS | TRP | GLU | CYS | PRO | TRP | HIS | HIS | CYS | ASP | ||||
| 7 | VAL | CYS | GLY | LYS | PRO | SER | THR | SER | PHE | CYS | ||||
| 8 | HIS | LEU | CYS | PRO | ASN | SER | PHE | CYS | LYS | GLU | ||||
| 9 | HIS | GLN | ASP | GLY | THR | ALA | PHE | SER | CYS | THR | ||||
| 10 | PRO | ASP | GLY | ARG | SER | TYR | CYS | SER | GLU | HIS | ||||
| 11 | ASP | LEU | GLY | ALA | ALA |
Entity 2, Zn_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHDvC5HCH, [U-99% 15N], 0.4 mM; Phosphate Buffer 20 mM; NaCl 150 mM; Tcep 2 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.3; pressure: 1 atm; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks