BMRB Entry 51636

Name: Backbone 1H, 13C and 15N Assignments of MYC(151-255)
Published: 2024-08-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51636

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Assignments of MYC(151-255)

Deposition date:

2022-09-20

Original release date:

2024-08-09

Authors:

Schutz, Stefan; Jahnke, Wolfgang

Citation:

Citation: Schutz, Stefan; Bergsdorf, Christian; Hanni-Holzinger, Sandra; Lingel, Andreas; Renatus, Martin; Gossert, Alvar; Jahnke, Wolfgang. "Intrinsically Disordered Regions in the Transcription Factor MYC:MAX Modulate DNA Binding via Intramolecular Interactions" Biochemistry ., .-. (2024).
PubMed: 38264995

Assembly members:

Assembly members:
entity_1, polymer, 105 residues, 10884.5 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-like

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SYQAARKDSGSPNPARGHSV CSTSSLYLQDLSAAASECID PSVVFPYPLNDSSSPKSCAS QDSSAFSPSSDSLLSSTESS PQGSPEPLVLHEETPPTTSS DSEEE

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	575
15N chemical shifts	125
1H chemical shifts	125

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MYC(151-255), trans- conformer	1
2	MYC(151-255), cis- conformer	1

Entities:

Entity 1, MYC(151-255), trans- conformer 105 residues - 10884.5 Da.

1

SER

TYR

GLN

ALA

ARG

LYS

ASP

SER

GLY

2

SER

PRO

ASN

PRO

ALA

ARG

GLY

HIS

SER

VAL

3

CYS

SER

THR

SER

LEU

TYR

LEU

GLN

ASP

4

LEU

SER

ALA

SER

GLU

CYS

ILE

ASP

5

PRO

SER

VAL

PHE

PRO

TYR

PRO

LEU

ASN

6

ASP

SER

PRO

LYS

SER

CYS

ALA

SER

7

GLN

ASP

SER

ALA

PHE

SER

PRO

SER

8

ASP

SER

LEU

SER

THR

GLU

SER

9

PRO

GLN

GLY

SER

PRO

GLU

PRO

LEU

VAL

LEU

10

HIS

GLU

THR

PRO

THR

SER

11

ASP

SER

GLU

Samples:

sample_1: MYC(256-351), [U-99% 13C; U-99% 15N], 0.665 mM; D2O, [U-99% 2H], 5 v/v; HEPES 20 mM; sodium chloride 100 mM; TCEP 2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2 - collection, data analysis, processing

CcpNMR v3.0 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks