BMRB Entry 51635

Title:
Backbone 1H, 13C and 15N Assignments of MYC(256-351)
Deposition date:
2022-09-20
Original release date:
2024-08-09
Authors:
Schutz, Stefan; Jahnke, Wolfgang
Citation:

Citation: Schutz, Stefan; Bergsdorf, Christian; Hanni-Holzinger, Sandra; Lingel, Andreas; Renatus, Martin; Gossert, Alvar; Jahnke, Wolfgang. "Intrinsically Disordered Regions in the Transcription Factor MYC:MAX Modulate DNA Binding via Intramolecular Interactions"  Biochemistry ., .-. (2024).
PubMed: 38264995

Assembly members:

Assembly members:
entity_1, polymer, 97 residues, 10682.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM41-like

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MYC(256-351), trans- conformer1
2MYC(256-351), cis- conformer1

Entities:

Entity 1, MYC(256-351), trans- conformer 97 residues - 10682.8 Da.

1   GLYGLNGLUASPGLUGLUGLUILEASPVAL
2   VALSERVALGLULYSARGGLNALAPROGLY
3   LYSARGSERGLUSERGLYSERPROSERALA
4   GLYGLYHISSERLYSPROPROHISSERPRO
5   LEUVALLEULYSARGCYSHISVALSERTHR
6   HISGLNHISASNTYRALAALAPROPROSER
7   THRARGLYSASPTYRPROALAALALYSARG
8   VALLYSLEUASPSERVALARGVALLEUARG
9   GLNILESERASNASNARGLYSCYSTHRSER
10   PROARGSERSERASPTHRGLU

Samples:

sample_1: MYC(256-351), [U-99% 13C; U-99% 15N], 0.9 mM; D2O, [U-99% 2H], 5 v/v; HEPES 20 mM; sodium chloride 100 mM; TCEP 2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection, data analysis, processing

CcpNMR v3.0 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks