BMRB Entry 51624

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51624
MolProbity Validation Chart

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Title:
CPSF73-CPSF100 C-terminal heterodimer
Deposition date:
2022-09-12
Original release date:
2023-02-08
Authors:
Thore, Stephane; Mackereth, Cameron
Citation:

Citation: Thore, Stephane; Fribourg, Sebastien; Mackereth, Cameron. "1H, 15N and 13C resonance assignments of a minimal CPSF73-CPSF100 C-terminal heterodimer"  Biomol. NMR Assignments 17, 43-48 (2023).
PubMed: 36723825

Assembly members:

Assembly members:
entity_1, polymer, 119 residues, Formula weight is not available
entity_2, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Encephalitozoon cuniculi   Taxonomy ID: 6035   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Encephalitozoon cuniculi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCN

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMLAKDNEAKIFTGVEGEF EGIIIGTEDDIRIYKRDELI ESKYKEMCIYERQRIPYNST AVLLKQVLVDGFEELVEVES GFMVGNVHIVLSGQEVVLEW KSSYTDDVLAISISKMISD
entity_2: MSDVSMGMVKLDKGFDALNY RAIGTDSVASFRGVRDGDMV RCIGEGPRMVIGHADINEMR RMIVEGSMRVEQEENGLLVE DCVWIRVSGDGVTIDGRDSG VFYAVRDVVYRSSAFI

Data sets:
Data typeCount
13C chemical shifts889
15N chemical shifts230
1H chemical shifts1572

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CPSF73 CTD1-CTD21
2CPSF100 CTD1-CTD22

Entities:

Entity 1, CPSF73 CTD1-CTD2 119 residues - Formula weight is not available

1   GLYHISMETLEUALALYSASPASNGLUALA
2   LYSILEPHETHRGLYVALGLUGLYGLUPHE
3   GLUGLYILEILEILEGLYTHRGLUASPASP
4   ILEARGILETYRLYSARGASPGLULEUILE
5   GLUSERLYSTYRLYSGLUMETCYSILETYR
6   GLUARGGLNARGILEPROTYRASNSERTHR
7   ALAVALLEULEULYSGLNVALLEUVALASP
8   GLYPHEGLUGLULEUVALGLUVALGLUSER
9   GLYPHEMETVALGLYASNVALHISILEVAL
10   LEUSERGLYGLNGLUVALVALLEUGLUTRP
11   LYSSERSERTYRTHRASPASPVALLEUALA
12   ILESERILESERLYSMETILESERASP

Entity 2, CPSF100 CTD1-CTD2 116 residues - Formula weight is not available

1   METSERASPVALSERMETGLYMETVALLYS
2   LEUASPLYSGLYPHEASPALALEUASNTYR
3   ARGALAILEGLYTHRASPSERVALALASER
4   PHEARGGLYVALARGASPGLYASPMETVAL
5   ARGCYSILEGLYGLUGLYPROARGMETVAL
6   ILEGLYHISALAASPILEASNGLUMETARG
7   ARGMETILEVALGLUGLYSERMETARGVAL
8   GLUGLNGLUGLUASNGLYLEULEUVALGLU
9   ASPCYSVALTRPILEARGVALSERGLYASP
10   GLYVALTHRILEASPGLYARGASPSERGLY
11   VALPHETYRALAVALARGASPVALVALTYR
12   ARGSERSERALAPHEILE

Samples:

sample_1: CPSF73, [U-99% 13C; U-99% 15N], 230 uM; CPSF100, [U-99% 13C; U-99% 15N], 230 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_2: CPSF73, [U-15N; 99% 13C-Ile], 625 uM; CPSF100, [U-15N; 99% 13C-Ile], 625 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_3: CPSF73, [U-15N; 99% 13C-Val], 150 uM; CPSF100, [U-15N; 99% 13C-Val], 150 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_4: CPSF73, [U-15N; 99% 13C-Leu], 280 uM; CPSF100, [U-15N; 99% 13C-Leu], 280 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_5: CPSF73, [U-10% 13C; U-100% 15N], 880 uM; CPSF100, [U-10% 13C; U-100% 15N], 880 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_6: CPSF73 458 uM; CPSF100 458 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_7: CPSF73, [95% 2H]-Phe, 150 uM; CPSF100, [95% 2H]-Phe, 150 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_8: CPSF73, [95% 2H]-Tyr,Trp, 85 uM; CPSF100, [95% 2H]-Tyr,Trp, 85 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 170 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HA(CACO)NHsample_1isotropicsample_conditions_1
3D N(COCA)NNHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D TROSY-HNCOsample_1isotropicsample_conditions_1
3D TROSY-HNCAsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_1isotropicsample_conditions_1
3D (H)C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C ct-HSQC methylsample_2isotropicsample_conditions_1
2D 1H-13C ct-HSQC methylsample_3isotropicsample_conditions_1
2D 1H-13C ct-HSQC methylsample_4isotropicsample_conditions_1
3D TROSY-HNCOsample_2isotropicsample_conditions_1
3D TROSY-HNCOsample_3isotropicsample_conditions_1
3D TROSY-HNCOsample_4isotropicsample_conditions_1
3D TROSY-HNCAsample_2isotropicsample_conditions_1
3D TROSY-HNCAsample_3isotropicsample_conditions_1
3D TROSY-HNCAsample_4isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_3isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_4isotropicsample_conditions_1
2D 1H-13C ct-HSQC methylsample_5isotropicsample_conditions_1
3D 1H-15N HSQC-TOCSYsample_5isotropicsample_conditions_1
2D 1H-1H NOESYsample_6isotropicsample_conditions_1
2D 1H-1H TOCSYsample_6isotropicsample_conditions_1
2D 1H-1H DQF-COSYsample_6isotropicsample_conditions_1
2D 1H-1H TOCSYsample_7isotropicsample_conditions_1
2D 1H-1H DQF-COSYsample_7isotropicsample_conditions_1
2D 1H-1H TOCSYsample_8isotropicsample_conditions_1
2D 1H-1H DQF-COSYsample_8isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_2isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_3isotropicsample_conditions_1
2D HN(CA)sample_2isotropicsample_conditions_1
2D HN(CA)sample_3isotropicsample_conditions_1
2D HN(CO)sample_2isotropicsample_conditions_1
2D HN(CO)sample_3isotropicsample_conditions_1
3D HSQC-NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0 - collection

NMRPipe v8.6 - processing

SPARKY vNMRFAM - data analysis

SPARKY vNMRFAM - peak picking

SPARKY vNMRFAM - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz
  • Bruker AVANCE NEO 800 MHz

Related Database Links:

GB KMV65242.1 NP_597379.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks