BMRB Entry 51617

Title:
Nish P3
Deposition date:
2022-09-06
Original release date:
2023-03-13
Authors:
Sethi, Ashish; Gooley, Paul; Dubey, Abhinav; Arthanari, Haribabu
Citation:

Citation: Sethi, Ashish; Rawlinson, Stephen; Dubey, Abhinav; Ang, Ching-Seng; Choi, Yoon Hee; Yan, Fei; Okada, Kazuma; Rozario, Ashley; Brice, Aaron; Ito, Naoto; Williamson, Nicholas; Hatters, Danny; Bell, Toby; Arthanari, Haribabu; Moseley, Gregory; Gooley, Paul. "Structural insights into the multifunctionality of rabies virus P3 protein"  Proc. Natl. Acad. Sci. U. S. A. 120, e2217066120-e2217066120 (2023).
PubMed: 36989298

Assembly members:

Assembly members:
entity_1, polymer, 297 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts202
15N chemical shifts66
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nish P3, chain 11
2Nish P3, chain 21

Entities:

Entity 1, Nish P3, chain 1 297 residues - Formula weight is not available

1   METSERLYSILEPHEVALASNPROSERALA
2   ILEARGALAGLYLEUALAASPLEUGLUMET
3   ALAGLUGLUTHRVALASPLEUILEASNARG
4   ASNILEGLUASPASNGLNALAHISLEUGLN
5   GLYGLUPROILEGLUVALASPSERLEUPRO
6   GLUASPMETSERARGLEUHISLEUASPASP
7   GLYLYSLEUPROASPLEUGLYARGMETSER
8   LYSALAGLYGLUGLYARGHISGLNGLUASP
9   PHEGLNMETASPGLUGLYGLUASPPROSER
10   LEULEUPHEGLNSERTYRLEUASPASNVAL
11   GLYVALGLNILEVALARGGLNMETARGSER
12   GLYGLUARGPHELEULYSILETRPSERGLN
13   THRVALGLUGLUILEILESERTYRVALTHR
14   VALASNPHEPROASNPROSERGLYARGSER
15   SERGLUASPLYSSERTHRGLNTHRTHRSER
16   GLNGLUPROLYSLYSGLUTHRTHRSERTHR
17   PROSERGLNARGLYSSERGLNSERLEULYS
18   SERARGTHRMETALAGLNTHRALASERGLY
19   PROPROSERLEUGLUTRPSERALATHRASN
20   GLUGLUASPASPLEUSERVALGLUALAGLU
21   ILEALAHISGLNILEALAGLUSERPHESER
22   LYSLYSTYRLYSPHEPROSERARGSERSER
23   GLYILEPHELEUTYRASNPHEGLUGLNLEU
24   LYSMETASNLEUASPASPILEVALLYSGLU
25   ALALYSASNVALPROGLYVALTHRARGLEU
26   ALAHISASPGLYSERLYSLEUPROLEUARG
27   CYSVALLEUGLYTRPVALALALEUALAASN
28   SERLYSLYSPHEGLNLEULEUVALGLUALA
29   ASNLYSLEUASNLYSILEMETGLNASPASP
30   LEUASNARGTYRALASERCYS

Samples:

sample_1: Nish P3, [U-100% 13C; U-100% 15N], 0.8 uM; potassium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks