BMRB Entry 51617

Name: Nish P3
Published: 2023-03-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51617

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Nish P3

Deposition date:

2022-09-06

Original release date:

2023-03-13

Authors:

Sethi, Ashish; Gooley, Paul; Dubey, Abhinav; Arthanari, Haribabu

Citation:

Citation: Sethi, Ashish; Rawlinson, Stephen; Dubey, Abhinav; Ang, Ching-Seng; Choi, Yoon Hee; Yan, Fei; Okada, Kazuma; Rozario, Ashley; Brice, Aaron; Ito, Naoto; Williamson, Nicholas; Hatters, Danny; Bell, Toby; Arthanari, Haribabu; Moseley, Gregory; Gooley, Paul. "Structural insights into the multifunctionality of rabies virus P3 protein" Proc. Natl. Acad. Sci. U. S. A. 120, e2217066120-e2217066120 (2023).
PubMed: 36989298

Assembly members:

Assembly members:
entity_1, polymer, 297 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSKIFVNPSAIRAGLADLEM AEETVDLINRNIEDNQAHLQ GEPIEVDSLPEDMSRLHLDD GKLPDLGRMSKAGEGRHQED FQMDEGEDPSLLFQSYLDNV GVQIVRQMRSGERFLKIWSQ TVEEIISYVTVNFPNPSGRS SEDKSTQTTSQEPKKETTST PSQRKSQSLKSRTMAQTASG PPSLEWSATNEEDDLSVEAE IAHQIAESFSKKYKFPSRSS GIFLYNFEQLKMNLDDIVKE AKNVPGVTRLAHDGSKLPLR CVLGWVALANSKKFQLLVEA NKLNKIMQDDLNRYASC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	202
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nish P3, chain 1	1
2	Nish P3, chain 2	1

Entities:

Entity 1, Nish P3, chain 1 297 residues - Formula weight is not available

1

MET

SER

LYS

ILE

PHE

VAL

ASN

PRO

SER

ALA

2

ILE

ARG

ALA

GLY

LEU

ALA

ASP

LEU

GLU

MET

3

ALA

GLU

THR

VAL

ASP

LEU

ILE

ASN

ARG

4

ASN

ILE

GLU

ASP

ASN

GLN

ALA

HIS

LEU

GLN

5

GLY

GLU

PRO

ILE

GLU

VAL

ASP

SER

LEU

PRO

6

GLU

ASP

MET

SER

ARG

LEU

HIS

LEU

ASP

7

GLY

LYS

LEU

PRO

ASP

LEU

GLY

ARG

MET

SER

8

LYS

ALA

GLY

GLU

GLY

ARG

HIS

GLN

GLU

ASP

9

PHE

GLN

MET

ASP

GLU

GLY

GLU

ASP

PRO

SER

10

LEU

PHE

GLN

SER

TYR

LEU

ASP

ASN

VAL

11

GLY

VAL

GLN

ILE

VAL

ARG

GLN

MET

ARG

SER

12

GLY

GLU

ARG

PHE

LEU

LYS

ILE

TRP

SER

GLN

13

THR

VAL

GLU

ILE

SER

TYR

VAL

THR

14

VAL

ASN

PHE

PRO

ASN

PRO

SER

GLY

ARG

SER

15

SER

GLU

ASP

LYS

SER

THR

GLN

THR

SER

16

GLN

GLU

PRO

LYS

GLU

THR

SER

THR

17

PRO

SER

GLN

ARG

LYS

SER

GLN

SER

LEU

LYS

18

SER

ARG

THR

MET

ALA

GLN

THR

ALA

SER

GLY

19

PRO

SER

LEU

GLU

TRP

SER

ALA

THR

ASN

20

GLU

ASP

LEU

SER

VAL

GLU

ALA

GLU

21

ILE

ALA

HIS

GLN

ILE

ALA

GLU

SER

PHE

SER

22

LYS

TYR

LYS

PHE

PRO

SER

ARG

SER

23

GLY

ILE

PHE

LEU

TYR

ASN

PHE

GLU

GLN

LEU

24

LYS

MET

ASN

LEU

ASP

ILE

VAL

LYS

GLU

25

ALA

LYS

ASN

VAL

PRO

GLY

VAL

THR

ARG

LEU

26

ALA

HIS

ASP

GLY

SER

LYS

LEU

PRO

LEU

ARG

27

CYS

VAL

LEU

GLY

TRP

VAL

ALA

LEU

ALA

ASN

28

SER

LYS

PHE

GLN

LEU

VAL

GLU

ALA

29

ASN

LYS

LEU

ASN

LYS

ILE

MET

GLN

ASP

30

LEU

ASN

ARG

TYR

ALA

SER

CYS

Samples:

sample_1: Nish P3, [U-100% 13C; U-100% 15N], 0.8 uM; potassium phosphate 50 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks