BMRB Entry 51614

Name: FUS ZnF Domain
Published: 2023-02-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51614

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FUS ZnF Domain

Deposition date:

2022-09-06

Original release date:

2023-02-27

Authors:

Felix, Sara; Oroz, Javier; Cabrita, Eurico; Laurents, Douglas

Citation:

Citation: Felix, Sara; Laurents, Douglas; Oroz, Javier; Cabrita, Eurico. "Fused in sarcoma undergoes cold denaturation: Implications for phase separation" Protein Sci. 32, e4521-e4521 (2023).
PubMed: 36453011

Assembly members:

Assembly members:
entity_1, polymer, 38 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGQQRAGDWKCPNPTCENMN FSWRNECNQCKAPKPDGX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	53
1H chemical shifts	158

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FUS ZnF	1
2	ZN	2

Entities:

Entity 1, FUS ZnF 38 residues - Formula weight is not available

1

ACE

GLY

GLN

ARG

ALA

GLY

ASP

TRP

LYS

2

CYS

PRO

ASN

PRO

THR

CYS

GLU

ASN

MET

ASN

3

PHE

SER

TRP

ARG

ASN

GLU

CYS

ASN

GLN

CYS

4

LYS

ALA

PRO

LYS

PRO

ASP

GLY

NH2

Entity 2, ZN - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: FUS ZnF 0.175 ± 0.01 mM; ZnCl2 2 ± 0.1 mM; deuterated sodium acetate 20 mM; sodium chloride 100 mM; DSS 0.05 mM

sample_5: FUS ZnF 0.923 ± 0.02 mM; ZnCl2 2 ± 0.1 mM; deuterated sodium acetate 20 mM; sodium chloride 100 mM; DSS 0.05 mM

sample_3: FUS ZnF 0.175 ± 0.01 mM; ZnCl2 2 ± 0.1 mM; deuterated sodium acetate 20 mM; sodium chloride 100 mM; DSS 0.05 mM

sample_conditions_1: ionic strength: 0.2481 M; pH: 6.53; pressure: 0.93 atm; temperature: 293.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_5	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_5	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.8 - collection

NMRFAM-SPARKY v1.4 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz