BMRB Entry 51612

Title:
Uracil-DNA glycosylase efficiency is modulated by substrate rigidity
Deposition date:
2022-09-02
Original release date:
2023-03-14
Authors:
Orndorff, Paul; Poddar, Souvik; Owens, Aerial; Kumari, Nikita; Ugaz, Bryan; Amin, Samrat; Van Horn, Wade; van der Vaart, Arjan; Levitus, Marcia
Citation:

Citation: Orndorff, Paul; Poddar, Souvik; Owens, Aerial; Kumari, Nikita; Ugaz, Bryan; Amin, Samrat; Van Horn, Wade; van der Vaart, Arjan; Levitus, Marcia. "Uracil-DNA glycosylase efficiency is modulated by substrate rigidity"  Sci. Rep. 13, 3915-3915 (2023).
PubMed: 36890276

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, Formula weight is not available
entity_2, polymer, 13 residues, Formula weight is not available
entity_3, polymer, 13 residues, Formula weight is not available
entity_4, polymer, 13 residues, Formula weight is not available
entity_5, polymer, 13 residues, Formula weight is not available
entity_6, polymer, 13 residues, Formula weight is not available
entity_7, polymer, 13 residues, Formula weight is not available
entity_8, polymer, 13 residues, Formula weight is not available
entity_9, polymer, 13 residues, Formula weight is not available
entity_10, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GTGAAAUTGTTAC
entity_2: GTAACAATTTCAC
entity_3: GTGAATUAGTTAC
entity_4: GTAACTAATTCAC
entity_5: GTGAAAUAGTTAC
entity_6: GTAACTATTTCAC
entity_7: GTGAATUTGTTAC
entity_8: GTAACAAATTCAC
entity_9: GTGCAAUTATGAC
entity_10: GTCATAATTGCAC

Data typeCount
H exchange rates38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
14ATF1
24ATR2
34TAF3
44TAR4
54AAF5
64AAR6
74TTF7
84TTR8
91ATF9
101ATR10

Entities:

Entity 1, 4ATF 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDGDADADADUDTDGDT
2   DTDADC

Entity 2, 4ATR 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDADADCDADADTDTDT
2   DCDADC

Entity 3, 4TAF 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDGDADADTDUDADGDT
2   DTDADC

Entity 4, 4TAR 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDADADCDTDADADTDT
2   DCDADC

Entity 5, 4AAF 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDGDADADADUDADGDT
2   DTDADC

Entity 6, 4AAR 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDADADCDTDADTDTDT
2   DCDADC

Entity 7, 4TTF 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDGDADADTDUDTDGDT
2   DTDADC

Entity 8, 4TTR 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDADADCDADADADTDT
2   DCDADC

Entity 9, 1ATF 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDGDCDADADUDTDADT
2   DGDADC

Entity 10, 1ATR 13 residues - Formula weight is not available

complement strand pairs A with U

1   DGDTDCDADTDADADTDTDG
2   DCDADC

Samples:

sample_1: 4ATF 3 mM; 4ATR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM

sample_2: 4TAF 3 mM; 4TAR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM

sample_3: 4AAF 3 mM; 4AAR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM

sample_4: 4TTF 3 mM; 4TTR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM

sample_5: 1ATF 3 mM; 1ATR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM

sample_conditions_1: pH: 7.0; temperature: 293.15 K

sample_conditions_2: pH: 7.0; temperature: 293.15 K

sample_conditions_3: pH: 7.0; temperature: 293.15 K

sample_conditions_4: pH: 7.0; temperature: 293.15 K

sample_conditions_5: pH: 7.0; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_3isotropicsample_conditions_3
2D 1H-1H NOESYsample_4isotropicsample_conditions_4
2D 1H-1H NOESYsample_5isotropicsample_conditions_5
T1/R1 relaxationsample_1isotropicsample_conditions_1
T1/R1 relaxationsample_2isotropicsample_conditions_2
T1/R1 relaxationsample_3isotropicsample_conditions_3
T1/R1 relaxationsample_4isotropicsample_conditions_4
T1/R1 relaxationsample_5isotropicsample_conditions_5

Software:

TOPSPIN v4.1 - chemical shift assignment, collection, processing

MATLAB v2019b - data analysis, processing

NMR spectrometers:

  • Bruker AVANCE III 850 MHz
  • Bruker AVANCE III 600 MHz