BMRB Entry 51611

Title:
1H, 13C and 15N Backbone Chemical Shift Assignments of NUT7
Deposition date:
2022-09-01
Original release date:
2022-11-29
Authors:
Salvi, Nicola; Ibrahim, Ziad; Blackledge, Martin; Panne, Daniel
Citation:

Citation: Ibrahim, Ziad; Wang, Tao; Destaing, Olivier; Salvi, Nicola; Hoghoughi, Naghmeh; Chabert, Clovis; Rusu, Alexandra; Gao, Jinjun; Feletto, Leonardo; Reynoird, Nicolas; Schalch, Thomas; Zhao, Yingming; Blackledge, Martin; Khochbin, Saadi; Panne, Daniel. "Structural insights into p300 regulation and acetylation-dependent genome organisation"  Nat. Commun. 13, 7759-7759 (2022).
PubMed: 36522330

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: -

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMAWPLALIEELEQEEGLT LAQLVQKRLMALEEEEDAEA PPSFSGAQLD

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts39
1H chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NUT71

Entities:

Entity 1, NUT7 50 residues - Formula weight is not available

1   GLYALAMETALATRPPROLEUALALEUILE
2   GLUGLULEUGLUGLNGLUGLUGLYLEUTHR
3   LEUALAGLNLEUVALGLNLYSARGLEUMET
4   ALALEUGLUGLUGLUGLUASPALAGLUALA
5   PROPROSERPHESERGLYALAGLNLEUASP

Samples:

sample_1: NUT7, [U-13C; U-15N], 500 uM; Tris 20 mM; NaCl 300 mM; DTT 1 mM; ZnSO4 5 uM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks