BMRB Entry 51610

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51610

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Title:
EcFTSZ_TEDOR_DNP
Deposition date:
2022-08-31
Original release date:
2022-09-21
Authors:
McCoy, Kelsey; Fritzsching, Keith; McDermott, Ann
Citation:

Citation: McCoy, Kelsey; Fritzsching, Keith; McDermott, Ann. "GTP-Bound Escherichia coli FtsZ Filaments Are Composed of Tense Monomers: a Dynamic Nuclear Polarization-Nuclear Magnetic Resonance Study Using Interface Detection"  mBio 13, e0235822-e0235822 (2022).
PubMed: 36214571

Assembly members:

Assembly members:
entity_1, polymer, 383 residues, Formula weight is not available
entity_GTP, non-polymer, 523.180 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MFEPMELTNDAVIKVIGVGG GGGNAVEHMVRERIEGVEFF AVNTDAQALRKTAVGQTIQI GSGITKGLGAGANPEVGRNA ADEDRDALRAALEGADMVFI AAGMGGGTGTGAAPVVAEVA KDLGILTVAVVTKPFNFEGK KRMAFAEQGITELSKHVDSL ITIPNDKLLKVLGRGISLLD AFGAANDVLKGAVQGIAELI TRPGLMNVDFADVRTVMSEM GYAMMGSGVASGEDRAEEAA EMAISSPLLEDIDLSGARGV LVNITAGFDLRLDEFETVGN TIRAFASDNATVVIGTSLDP DMNDELRVTVVATGIGMDKR PEITLVTNKQVQQPVMDRYQ QHGMAPLTQEQKPVAKVVND NAPQTAKEPDYLDIPAFLRK QAD

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FtsZ filament1
2GTP2

Entities:

Entity 1, FtsZ filament 383 residues - Formula weight is not available

1   METPHEGLUPROMETGLULEUTHRASNASP
2   ALAVALILELYSVALILEGLYVALGLYGLY
3   GLYGLYGLYASNALAVALGLUHISMETVAL
4   ARGGLUARGILEGLUGLYVALGLUPHEPHE
5   ALAVALASNTHRASPALAGLNALALEUARG
6   LYSTHRALAVALGLYGLNTHRILEGLNILE
7   GLYSERGLYILETHRLYSGLYLEUGLYALA
8   GLYALAASNPROGLUVALGLYARGASNALA
9   ALAASPGLUASPARGASPALALEUARGALA
10   ALALEUGLUGLYALAASPMETVALPHEILE
11   ALAALAGLYMETGLYGLYGLYTHRGLYTHR
12   GLYALAALAPROVALVALALAGLUVALALA
13   LYSASPLEUGLYILELEUTHRVALALAVAL
14   VALTHRLYSPROPHEASNPHEGLUGLYLYS
15   LYSARGMETALAPHEALAGLUGLNGLYILE
16   THRGLULEUSERLYSHISVALASPSERLEU
17   ILETHRILEPROASNASPLYSLEULEULYS
18   VALLEUGLYARGGLYILESERLEULEUASP
19   ALAPHEGLYALAALAASNASPVALLEULYS
20   GLYALAVALGLNGLYILEALAGLULEUILE
21   THRARGPROGLYLEUMETASNVALASPPHE
22   ALAASPVALARGTHRVALMETSERGLUMET
23   GLYTYRALAMETMETGLYSERGLYVALALA
24   SERGLYGLUASPARGALAGLUGLUALAALA
25   GLUMETALAILESERSERPROLEULEUGLU
26   ASPILEASPLEUSERGLYALAARGGLYVAL
27   LEUVALASNILETHRALAGLYPHEASPLEU
28   ARGLEUASPGLUPHEGLUTHRVALGLYASN
29   THRILEARGALAPHEALASERASPASNALA
30   THRVALVALILEGLYTHRSERLEUASPPRO
31   ASPMETASNASPGLULEUARGVALTHRVAL
32   VALALATHRGLYILEGLYMETASPLYSARG
33   PROGLUILETHRLEUVALTHRASNLYSGLN
34   VALGLNGLNPROVALMETASPARGTYRGLN
35   GLNHISGLYMETALAPROLEUTHRGLNGLU
36   GLNLYSPROVALALALYSVALVALASNASP
37   ASNALAPROGLNTHRALALYSGLUPROASP
38   TYRLEUASPILEPROALAPHELEUARGLYS
39   GLNALAASP

Entity 2, GTP - C10 H16 N5 O14 P3 - 523.180 Da.

1   GTP

Samples:

sample_1: EcFtsZ, 2-[13C]-glycerol, 2.5 mg; EcFtsZ, [U-15N, 12C], 2.5 mg; GTP 5 mM; KCl 25 mM; MgCl2 5 mM; MES 50 mM; EGTA 1 mM; glycerol, [U-12C], 30%

sample_2: EcFtsZ, 1,3-[13C]-glycerol, 2.5 mg; EcFtsZ, [U-15N, 12C], 2.5 mg; GTP 5 mM; KCl 25 mM; MgCl2 5 mM; MES 50 mM; EGTA 1 mM; glycerol, [U-12C], 30%

sample_3: EcFtsZ, 1,3-[13C]-glycerol, 2.5 mg; EcFtsZ, [U-15N], 2.5 mg; GTP 5 mM; KCl 25 mM; MgCl2 5 mM; MES 50 mM; EGTA 1 mM; glycerol, [U-12C], 30%

sample_4: EcFtsZ, [13Ca]-GLY, [13Cb]-ALA, 2.5 mg; EcFtsZ, [U-15N, 12C], 2.5 mg; GTP 5 mM; KCl 25 mM; MgCl2 5 mM; MES 50 mM; EGTA 1 mM; glycerol, [U-12C], 30%

sample_5: EcFtsZ, [U-15N], 5 mg; GTP 5 mM; KCl 25 mM; MgCl2 5 mM; MES 50 mM; EGTA 1 mM; glycerol, [U-12C], 30%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 115 K

Experiments:

NameSampleSample stateSample conditions
13C CPsample_1isotropicsample_conditions_1
1D ZF-TEDORsample_1isotropicsample_conditions_1
13C CPsample_2isotropicsample_conditions_1
1D ZF-TEDORsample_2isotropicsample_conditions_1
13C CPsample_3isotropicsample_conditions_1
1D ZF-TEDORsample_3isotropicsample_conditions_1
13C CPsample_4isotropicsample_conditions_1
1D ZF-TEDORsample_4isotropicsample_conditions_1
13C CPsample_5isotropicsample_conditions_1
1D ZF-TEDORsample_5isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III 600 MHz