BMRB Entry 51606

Title:
KH1-2 Fragile X mental retardation protein D331-396
Deposition date:
2022-08-31
Original release date:
2023-12-19
Authors:
Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Ramos, Andres
Citation:

Citation: Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Kelly, Geoff; Christodoulou, Evangelios; Taylor, Ian; Ramos, Andres. "Affinity-enhanced RNA-binding domains as tools to understand RNA recognition"  Cell Rep. Methods 3, 100508-100508 (2023).
PubMed: 37426752

Assembly members:

Assembly members:
entity_1, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-47b

Data sets:
Data typeCount
13C chemical shifts174
15N chemical shifts94
1H chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Didomain KH1KH21

Entities:

Entity 1, Didomain KH1KH2 144 residues - Formula weight is not available

1   ALASERARGPHEHISGLUGLNPHEILEVAL
2   ARGGLUASPLEUMETGLYLEUALAILEGLY
3   THRHISGLYALAASNILEGLNGLNALAARG
4   LYSVALPROGLYVALTHRALAILEASPLEU
5   ASPGLUASPTHRCYSTHRPHEHISILETYR
6   GLYGLUASPGLNASPALAVALLYSLYSALA
7   ARGSERPHELEUGLUPHEALAGLUASPVAL
8   ILEGLNVALPROARGASNLEUVALGLYLYS
9   VALILEGLYLYSASNGLYLYSLEUILEGLN
10   GLUILEVALASPLYSSERGLYVALVALARG
11   VALARGILEGLUALAGLUASNGLULYSASN
12   VALPROGLNGLUGLUGLYMETVALPROPHE
13   VALPHEVALGLYTHRLYSASPSERILEALA
14   ASNALATHRVALLEULEUASPTYRHISLEU
15   ASNTYRLEULYS

Samples:

sample_1: Fragile X mental retardation protein D331-396, [U-13C; U-15N; U-2H], 200 uM

sample_conditions_1: pH: 6.9; temperature: 301.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks