BMRB Entry 51606

Name: KH1-2 Fragile X mental retardation protein D331-396
Published: 2023-12-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51606

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

KH1-2 Fragile X mental retardation protein D331-396

Deposition date:

2022-08-31

Original release date:

2023-12-19

Authors:

Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Ramos, Andres

Citation:

Citation: Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Kelly, Geoff; Christodoulou, Evangelios; Taylor, Ian; Ramos, Andres. "Affinity-enhanced RNA-binding domains as tools to understand RNA recognition" Cell Rep. Methods 3, 100508-100508 (2023).
PubMed: 37426752

Assembly members:

Assembly members:
entity_1, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ASRFHEQFIVREDLMGLAIG THGANIQQARKVPGVTAIDL DEDTCTFHIYGEDQDAVKKA RSFLEFAEDVIQVPRNLVGK VIGKNGKLIQEIVDKSGVVR VRIEAENEKNVPQEEGMVPF VFVGTKDSIANATVLLDYHL NYLK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	174
15N chemical shifts	94
1H chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Didomain KH1KH2	1

Entities:

Entity 1, Didomain KH1KH2 144 residues - Formula weight is not available

1

ALA

SER

ARG

PHE

HIS

GLU

GLN

PHE

ILE

VAL

2

ARG

GLU

ASP

LEU

MET

GLY

LEU

ALA

ILE

GLY

3

THR

HIS

GLY

ALA

ASN

ILE

GLN

ALA

ARG

4

LYS

VAL

PRO

GLY

VAL

THR

ALA

ILE

ASP

LEU

5

ASP

GLU

ASP

THR

CYS

THR

PHE

HIS

ILE

TYR

6

GLY

GLU

ASP

GLN

ASP

ALA

VAL

LYS

ALA

7

ARG

SER

PHE

LEU

GLU

PHE

ALA

GLU

ASP

VAL

8

ILE

GLN

VAL

PRO

ARG

ASN

LEU

VAL

GLY

LYS

9

VAL

ILE

GLY

LYS

ASN

GLY

LYS

LEU

ILE

GLN

10

GLU

ILE

VAL

ASP

LYS

SER

GLY

VAL

ARG

11

VAL

ARG

ILE

GLU

ALA

GLU

ASN

GLU

LYS

ASN

12

VAL

PRO

GLN

GLU

GLY

MET

VAL

PRO

PHE

13

VAL

PHE

VAL

GLY

THR

LYS

ASP

SER

ILE

ALA

14

ASN

ALA

THR

VAL

LEU

ASP

TYR

HIS

LEU

15

ASN

TYR

LEU

LYS

Samples:

sample_1: Fragile X mental retardation protein D331-396, [U-13C; U-15N; U-2H], 200 uM

sample_conditions_1: pH: 6.9; temperature: 301.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks