BMRB Entry 51601

Name: BCATcterm NMR assignments
Published: 2023-06-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51601

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BCATcterm NMR assignments

Deposition date:

2022-08-29

Original release date:

2023-06-23

Authors:

Langelaan, David; Brown, Alexandra; Cranstone, Connor

Citation:

Citation: Brown, Alexandra; Cranstone, Connor; Dupre, Denis; Langelaan, David. "B-catenin interacts with the TAZ1 and TAZ2 domains of CBP/p300 to activate gene transcription" Int. J. Biol. Macromol. 238, 124155-124155 (2023).
PubMed: 36963539

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKPQDYKKRLSVELTSSLF RTEPMAWNETADLGLDIGAQ GEPLGYRQDDPSYRSFHSGG YGQDALGMDPMMEHEMGGHH PGADYPVDGLPDLGHAQDLM DGLPPGDSNQLAWFDTDL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	328
15N chemical shifts	105
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BCAT	1

Entities:

Entity 1, BCAT 118 residues - Formula weight is not available

1

GLY

SER

LYS

PRO

GLN

ASP

TYR

LYS

ARG

2

LEU

SER

VAL

GLU

LEU

THR

SER

LEU

PHE

3

ARG

THR

GLU

PRO

MET

ALA

TRP

ASN

GLU

THR

4

ALA

ASP

LEU

GLY

LEU

ASP

ILE

GLY

ALA

GLN

5

GLY

GLU

PRO

LEU

GLY

TYR

ARG

GLN

ASP

6

PRO

SER

TYR

ARG

SER

PHE

HIS

SER

GLY

7

TYR

GLY

GLN

ASP

ALA

LEU

GLY

MET

ASP

PRO

8

MET

GLU

HIS

GLU

MET

GLY

HIS

9

PRO

GLY

ALA

ASP

TYR

PRO

VAL

ASP

GLY

LEU

10

PRO

ASP

LEU

GLY

HIS

ALA

GLN

ASP

LEU

MET

11

ASP

GLY

LEU

PRO

GLY

ASP

SER

ASN

GLN

12

LEU

ALA

TRP

PHE

ASP

THR

ASP

LEU

Samples:

sample_1: BCAT666-781, [U-99% 13C; U-99% 15N], 500 uM; D2O 5%; DTT 5 mM; MES 20 mM; NaCl 100 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks