BMRB Entry 51598

Title:
SNAP25a (Rattus norvegicus)
Deposition date:
2022-08-24
Original release date:
2022-08-31
Authors:
Stief, Tobias; Lakomek, Nils-Alexander
Citation:

Citation: Stief, Tobias; Gremer, Lothar; Pribicevic, Sonja; Espinueva, Delane; Vormann, Katharina; Biehl, Ralf; Jahn, Reinhard; Perez-Lara, Angel; Lakomek, Nils-Alexander. "Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation"  J. Mol. Biol. 435, 168069-168069 (2023).
PubMed: 37003471

Assembly members:

Assembly members:
entity_1, polymer, 206 residues, 23272 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a(+)

Data sets:
Data typeCount
13C chemical shifts595
15N chemical shifts204
1H chemical shifts204

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SNAP25a1

Entities:

Entity 1, SNAP25a 206 residues - 23272 Da.

1   METALAGLUASPALAASPMETARGASNGLU
2   LEUGLUGLUMETGLNARGARGALAASPGLN
3   LEUALAASPGLUSERLEUGLUSERTHRARG
4   ARGMETLEUGLNLEUVALGLUGLUSERLYS
5   ASPALAGLYILEARGTHRLEUVALMETLEU
6   ASPGLUGLNGLYGLUGLNLEUASPARGVAL
7   GLUGLUGLYMETASNHISILEASNGLNASP
8   METLYSGLUALAGLULYSASNLEULYSASP
9   LEUGLYLYSSERSERGLYLEUPHEILESER
10   PROSERASNLYSLEULYSSERSERASPALA
11   TYRLYSLYSALATRPGLYASNASNGLNASP
12   GLYVALVALALASERGLNPROALAARGVAL
13   VALASPGLUARGGLUGLNMETALAILESER
14   GLYGLYPHEILEARGARGVALTHRASNASP
15   ALAARGGLUASNGLUMETASPGLUASNLEU
16   GLUGLNVALSERGLYILEILEGLYASNLEU
17   ARGHISMETALALEUASPMETGLYASNGLU
18   ILEASPTHRGLNASNARGGLNILEASPARG
19   ILEMETGLULYSALAASPSERASNLYSTHR
20   ARGILEASPGLUALAASNGLNARGALATHR
21   LYSMETLEUGLYSERGLY

Samples:

sample_1: SNAP25a, [U-99% 13C; U-99% 15N], 150 uM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 288.15 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - processing

CcpNMR v3 - peak picking

NMR spectrometers:

  • Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks