BMRB Entry 51593
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51593
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Citation: Escobedo, Albert; Piccirillo, Jonathan; Aranda, Juan; Diercks, Tammo; Mateos, Borja; Garcia-Cabau, Carla; Sanchez-Navarro, Macarena; Topal, Busra; Biesaga, Mateusz; Staby, Lasse; Kragelund, Birthe; Garcia, Jesus; Millet, Oscar; Orozco, Modesto; Coles, Murray; Crehuet, Ramon; Salvatella, Xavier. "A glutamine-based single alpha-helix scaffold to target globular proteins" Nat. Commun. 13, 7073-7073 (2022).
PubMed: 36400768
Assembly members:
entity_1, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KKPGASLALAQAQAAAKKY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 27 |
| 1H chemical shifts | 52 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P2-6 | 1 |
Entities:
Entity 1, P2-6 19 residues - Formula weight is not available
| 1 | LYS | LYS | PRO | GLY | ALA | SER | LEU | ALA | LEU | ALA | ||||
| 2 | GLN | ALA | GLN | ALA | ALA | ALA | LYS | LYS | TYR |
Samples:
sample_1: P2-6 100 uM; DSS 10 uM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 120 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN - collection, processing
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
- Bruker AVANCE III 600 MHz