BMRB Entry 5157

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PDB ID: 1j5b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5157
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein

Deposition date:

2001-09-24

Original release date:

2008-07-16

Authors:

Liepinsh, Edvards; Otting, Gottfried; Harding, Margaret; Ward, Leanne; Mackay, Joel; Haymet, A.

Citation:

Citation: Liepinsh, Edvards; Otting, Gottfried; Harding, Margaret; Ward, Leanne; Mackay, Joel; Haymet, A.. "Solution Structure of a Hydrophobic Analogue of the Winter Flounder Antifreeze Protein " Eur. J. Biochem. 269, 1259-1266 (2002).
PubMed: 11856360

Assembly members:

Assembly members:
antifreeze protein type 1 mutant, polymer, 37 residues, 3464 Da.

Natural source:

Natural source: Common Name: winter flounder Taxonomy ID: 8265 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Pseudopleuronectes americanus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
antifreeze protein type 1 mutant: DVASDAKAAAELVAANAKAA AELVAANAKAAAEAVAX

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	161

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	type 1 AFP mutant	1

Entities:

Entity 1, type 1 AFP mutant 37 residues - 3464 Da.

1

ASP

VAL

ALA

SER

ASP

ALA

LYS

ALA

2

GLU

LEU

VAL

ALA

ASN

ALA

LYS

ALA

3

ALA

GLU

LEU

VAL

ALA

ASN

ALA

LYS

ALA

4

ALA

GLU

ALA

VAL

ALA

AAR

Samples:

sample_1: antifreeze protein type 1 mutant 11 mM; NaCl 100 mM

sample_2: antifreeze protein type 1 mutant 2 mM; NaCl 100 mM

ex-cond_1: pH: 4.9; temperature: 288 K; ionic strength: 0.1 M

ex-cond_2: pH: 7.0; temperature: 283 K; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D ROESY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D DQF-COSY	not available	not available	not available

Software:

PROSA v2.6 - spectra processing

XEASY v2.5 - spectral analysis

DYANA v2.6 - structure calculation

OPAL v2.2 - structure refinement

NMR spectrometers:

Bruker DMX 600 MHz
Varian Unity-INOVA 800 MHz