BMRB Entry 5156

Title:
Partial assignment of the calcium-bound calretinin I-II domain (residues 1-100): 1H, 13C and 15N backbone assignments (and partial sidechain assignment) for structured elements
Deposition date:
2001-09-23
Original release date:
2002-01-25
Authors:
Palczewska, Malgorzata; Groves, Patrick; Ambrus, Attila; Kaleta, Agata; Kover, Katalin; Batta, Gyula; Kuznicki, Jacek
Citation:

Citation: Palczewska, Malgorzata; Groves, Patrick; Ambrus, Attila; Kaleta, Agata; Kover, Katalin; Batta, Gyula; Kuznicki, Jacek. "Structural and Biochemical Characterization of Neuronal Calretinin Domain I-II (residues 1-100). Comparison to Homologous Calbindin D28k Domain I-II (residues 1-93)"  Eur. J. Biochem. 268, 6229-6237 (2001).
PubMed: 11733019

Assembly members:

Assembly members:
calretinin, polymer, 104 residues, 12500 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts533
13C chemical shifts495
15N chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calretinin I-II domain1
2calcium ion, I2
3calcium ion, II2

Entities:

Entity 1, calretinin I-II domain 104 residues - 12500 Da.

1   TYRVALGLUPHEMETALAGLYPROGLNGLN
2   GLNPROPROTYRLEUHISLEUALAGLULEU
3   THRALASERGLNPHELEUGLUILETRPLYS
4   HISPHEASPALAASPGLYASNGLYTYRILE
5   GLUGLYLYSGLULEUGLUASNPHEPHEGLN
6   GLULEUGLULYSALAARGLYSGLYSERGLY
7   METMETSERLYSSERASPASNPHEGLYGLU
8   LYSMETLYSGLUPHEMETGLNLYSTYRASP
9   LYSASNSERASPGLYLYSILEGLUMETALA
10   GLULEUALAGLNILELEUPROTHRGLUGLU
11   ASNPHELEULEU

Entity 2, calcium ion, I - Ca - 40.078 Da.

1   CA

Samples:

sample_1: calretinin, [U-15N], 1 mM; TRIS, [U-2H], 50 mM; NaCl 25 mM; CaCl2 10 mM

sample_2: calretinin, [U-13C; U-15N], 1 mM; TRIS, [U-2H], 50 mM; NaCl 25 mM; CaCl2 10 mM

sample_cond_1: pH: 7.7; temperature: 303 K

sample_cond_2: pH: 6.7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
(Acq. points / acq. time nucleus Size of processed data (LP/ linear prediction))not availablenot availablenot available
experiment Dimension 1 Dimension 2 Dimension 3 Bruker pulse programnot availablenot availablenot available
1H -15N HSQC (128 / 90.2 ms 15N 192 LP /512) (512/ 102 ms 1H/1024)not availablenot availablenot available
invieagssi[ref-2; ref-3; ref-4];not availablenot availablenot available
HNCO (48 / 21.2 ms 13C 72 LP / 128) (34 / 24 ms 15N 52 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) hncogs3d.2[ref-5; ref-6];not availablenot availablenot available
HN(CA)CO (48 / 21.2 ms 13C 72 LP / 128) (34 / 24 ms 15N 52 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) hncacogs3d[ref-7; ref-8];not availablenot availablenot available
HNCA (128 / 25.4 ms 13C 128) (34 / 24 ms 15N 52 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) hncags3d.2[ref-5];not availablenot availablenot available
HN(CO)CA (84 / 59.3 ms 15N 128) (64 / 12.7 ms 13C 96 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) [ref-9];not availablenot availablenot available
HNCACB (64 / 7.27 ms 13C 96 LP / 128) (34 / 24 ms 15N 52 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) hncacbgs3d[ref-10; ref-11];not availablenot availablenot available
CBCANH (52 / 5.91 ms 13C 96 LP / 128) (34 / 24 ms 15N 52 LP / 128)not availablenot availablenot available
(1024 / 79 ms 1H 1024) cbcanhgs3d[ref-10; ref-12; ref-13];not availablenot availablenot available
CBCA(CO)NH (84 / 59.2 ms 15N 100 LP / 128) (70 / 7.95 ms 13C 100 LP / 128)not availablenot availablenot available
HBHA(CO)NH (76 / 53.5 ms 15N 100 LP / 128) (100 / 30.6 ms 1H 150 LP/ 256)not availablenot availablenot available
HNHA (64 / 45.1 ms 15N 100 LP / 128) (128 / 19.7 ms 1H 192 LP / 256)not availablenot availablenot available
(1024 / 79 ms 1H 1024) [ref-14];not availablenot availablenot available
(H)CC(CO)NH (84 / 59.2 ms 15N 100 LP / 128) (62 / 7.04 ms 13C 90 LP / 128)not availablenot availablenot available
H(CC)(CO)NH (76 / 53.5 ms 15N 100 LP / 128) (66 / 18.8 ms 1H 96 LP / 128)not availablenot availablenot available
NOESY-HSQC 15N and 13C (104 / 16 ms 1H 128 LP / 256)not availablenot availablenot available
(64 / 7.04 ms 13C 90 LP / 128) (1024 / 79 ms 1H 1024)not availablenot availablenot available
noesiisism3d[ref-15];not availablenot availablenot available
NOESY-HSQC 15N (104 / 16 ms 1H 128 LP / 256) (64 / 45.1 ms 15N 100 LP / 128)not availablenot availablenot available
(512 / 102 ms 1H 1024) noesiietf3gsrdf[ref-5].not availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

EMBL CAA39992
GB EDL92533
REF XP_002802612 XP_004782213 XP_006742579 XP_008519580 XP_009429507

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks