BMRB Entry 51550

Name: MITF81-204 NMR assignments
Published: 2023-06-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51550

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MITF81-204 NMR assignments

Deposition date:

2022-08-09

Original release date:

2023-06-29

Authors:

Langelaan, David; Brown, Alexandra; Branch, Makenzie

Citation:

Citation: Brown, Alexandra; Vergunst, Kathleen; Branch, Makenzie; Blair, Connor; Dupre, Denis; Ballie, George; Langelaan, David. "Structural basis of CBP/p300 recruitment by the microphthalmia-associated transcription factor" Biochim. Biophys. Acta Mol. Cell Res. 1870, 119520-119520 (2023).
PubMed: 37353163

Assembly members:

Assembly members:
entity_1, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSNSNCENSNCEKEGFYKFE EQNRAESECPGMNTHSRASC MQMDDVIDDIISLESSYNEE ILGLMDPALQMANTLPVSGN LIDLYGNQGLPPPGLTISNS CPANLPNIKRELTACIFPTE SEARALAKERQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	114
1H chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MITF	1

Entities:

Entity 1, MITF 131 residues - Formula weight is not available

1

GLY

SER

ASN

SER

ASN

CYS

GLU

ASN

SER

ASN

2

CYS

GLU

LYS

GLU

GLY

PHE

TYR

LYS

PHE

GLU

3

GLU

GLN

ASN

ARG

ALA

GLU

SER

GLU

CYS

PRO

4

GLY

MET

ASN

THR

HIS

SER

ARG

ALA

SER

CYS

5

MET

GLN

MET

ASP

VAL

ILE

ASP

ILE

6

ILE

SER

LEU

GLU

SER

TYR

ASN

GLU

7

ILE

LEU

GLY

LEU

MET

ASP

PRO

ALA

LEU

GLN

8

MET

ALA

ASN

THR

LEU

PRO

VAL

SER

GLY

ASN

9

LEU

ILE

ASP

LEU

TYR

GLY

ASN

GLN

GLY

LEU

10

PRO

GLY

LEU

THR

ILE

SER

ASN

SER

11

CYS

PRO

ALA

ASN

LEU

PRO

ASN

ILE

LYS

ARG

12

GLU

LEU

THR

ALA

CYS

ILE

PHE

PRO

THR

GLU

13

SER

GLU

ALA

ARG

ALA

LEU

ALA

LYS

GLU

ARG

14

GLN

Samples:

sample_1: MITF 81-204, [U-99% 13C; U-99% 15N], 400 uM; D2O 5%; DTT 5 mM; MES 20 mM; NaCl 50 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks