BMRB Entry 51548
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51548
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Citation: Singh, Himanshu. "Near complete 1H, 15N and 13C resonance assignments of the wild-type MBD from MeCP2" .
Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: SLEDRGPMYDDPTLPEGWTR
KLKQRKSGRSAGKYDVYLIN
PQGKAFRSKVELIAYFEKVG
DTSLDPNDFDFTVTGRGSPS
RREQKPPKKPKSPKATSHHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 455 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MeCP2 | 1 |
Entities:
Entity 1, MeCP2 104 residues - Formula weight is not available
| 1 | SER | LEU | GLU | ASP | ARG | GLY | PRO | MET | TYR | ASP | ||||
| 2 | ASP | PRO | THR | LEU | PRO | GLU | GLY | TRP | THR | ARG | ||||
| 3 | LYS | LEU | LYS | GLN | ARG | LYS | SER | GLY | ARG | SER | ||||
| 4 | ALA | GLY | LYS | TYR | ASP | VAL | TYR | LEU | ILE | ASN | ||||
| 5 | PRO | GLN | GLY | LYS | ALA | PHE | ARG | SER | LYS | VAL | ||||
| 6 | GLU | LEU | ILE | ALA | TYR | PHE | GLU | LYS | VAL | GLY | ||||
| 7 | ASP | THR | SER | LEU | ASP | PRO | ASN | ASP | PHE | ASP | ||||
| 8 | PHE | THR | VAL | THR | GLY | ARG | GLY | SER | PRO | SER | ||||
| 9 | ARG | ARG | GLU | GLN | LYS | PRO | PRO | LYS | LYS | PRO | ||||
| 10 | LYS | SER | PRO | LYS | ALA | THR | SER | HIS | HIS | HIS | ||||
| 11 | HIS | HIS | HIS | HIS |
Samples:
sample_1: Wild-type MBD from MeCP2, [U-100% 13C; U-100% 15N], 0.7 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 291 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks