BMRB Entry 51547

Title:
Near complete 1H, 15N and 13C resonance assignments of the MBD double mutant
Deposition date:
2022-08-04
Original release date:
2022-08-12
Authors:
Singh, Himanshu
Citation:

Citation: Singh, Himanshu. "Complete 1H, 15N and 13C assignments of the MBD double mutant"  .

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts82
1H chemical shifts493

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBD1

Entities:

Entity 1, MBD 104 residues - Formula weight is not available

1   SERLEUGLUASPARGGLYPROMETTYRASP
2   ASPPROTHRLEUPROGLUGLYTRPTHRARG
3   LYSLEUTHRGLNARGLYSSERGLYARGSER
4   ALAGLYLYSTYRASPVALTYRLEUILEASN
5   PROGLNGLYLYSALAPHEARGASNLYSVAL
6   GLULEUILEALATYRPHEGLULYSVALGLY
7   ASPTHRSERLEUASPPROASNASPPHEASP
8   PHETHRVALTHRGLYARGGLYSERPROSER
9   ARGARGGLUGLNLYSPROPROLYSLYSPRO
10   LYSSERPROLYSALATHRSERHISHISHIS
11   HISHISHISHIS

Samples:

sample_1: MBD, [U-100% 13C; U-100% 15N], 0.6 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 291; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks