BMRB Entry 51542

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51542

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Title:
1H, 13C and 15N resonance assignments for the BRCT domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei
Deposition date:
2022-08-02
Original release date:
2024-03-20
Authors:
Ludzia, Patryk; Robinson, Timothy; Akiyoshi, Bungo; Redfield, Christina
Citation:

Citation: Ludzia, Patryk; Hayashi, Hanako; Robinson, Timothy; Akiyoshi, Bungo; Redfield, Christina. "NMR study of the structure and dynamics of the BRCT domain from the kinetochore protein KKT4"  Biomol. NMR Assignments 18, 15-25 (2024).
PubMed: 38453826

Assembly members:

Assembly members:
entity_1, polymer, 185 residues, 19889.60 Da.

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMSGASSAVGGSTRSPSPVD PKRGAVQPRYFITTSLTEKE RNSVMEAIQKLGQRAVLVDN KVDEILPLNTTHIVLRGPPR SVKALCGVVSSKWLVQPSYV FDSLGAGFWLDEEVEGGLRY FPPPLRCQRFLLTMPEGVVK TMLQRVVEFGGGEVVGTKRN GSSNDQDVVVVSSGDELLRF AISRD

Data sets:
Data typeCount
13C chemical shifts642
15N chemical shifts180
1H chemical shifts988

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KKT41

Entities:

Entity 1, KKT4 185 residues - 19889.60 Da.

Residues 1-2 (461S, 462M) represent part of a remained linker after TEV protease cleavage.

1   SERMETSERGLYALASERSERALAVALGLY
2   GLYSERTHRARGSERPROSERPROVALASP
3   PROLYSARGGLYALAVALGLNPROARGTYR
4   PHEILETHRTHRSERLEUTHRGLULYSGLU
5   ARGASNSERVALMETGLUALAILEGLNLYS
6   LEUGLYGLNARGALAVALLEUVALASPASN
7   LYSVALASPGLUILELEUPROLEUASNTHR
8   THRHISILEVALLEUARGGLYPROPROARG
9   SERVALLYSALALEUCYSGLYVALVALSER
10   SERLYSTRPLEUVALGLNPROSERTYRVAL
11   PHEASPSERLEUGLYALAGLYPHETRPLEU
12   ASPGLUGLUVALGLUGLYGLYLEUARGTYR
13   PHEPROPROPROLEUARGCYSGLNARGPHE
14   LEULEUTHRMETPROGLUGLYVALVALLYS
15   THRMETLEUGLNARGVALVALGLUPHEGLY
16   GLYGLYGLUVALVALGLYTHRLYSARGASN
17   GLYSERSERASNASPGLNASPVALVALVAL
18   VALSERSERGLYASPGLULEULEUARGPHE
19   ALAILESERARGASP

Related Database Links:

UNP Q580Y8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks