BMRB Entry 51539

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51539

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Title:
Backbone assignment for Functional Amyloid in pseudomonas FapC
Deposition date:
2022-08-01
Original release date:
2023-06-30
Authors:
Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Matthews, Steve
Citation:

Citation: Rasmussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC"  J. Mol. Biol. 435, 167878-167878 (2023).
PubMed: 36368411

Assembly members:

Assembly members:
entity_1, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGPAEKWKPTPAPTGTVAAA VTDTQVSKDNKFDDTKTLNN AGANGSLSNSKGNLGANIAV GSGNQQDNAAAITSSAGDAA TVFAVADIYQQSKDNKFTNK GTQNNALLNNSANNSSGNVG VNVAAGQGNQQKNNLAIVTA NGKNVAAASNTEQVSLDNHF LNEASSKHSYKPQYVVNNAG LLNSANNASGNIGVNVAAGA GNQQSNTLTLGSGCTVCAAG TGSKLAFLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts394
15N chemical shifts215
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FapC1

Entities:

Entity 1, FapC 235 residues - Formula weight is not available

1   METGLYPROALAGLULYSTRPLYSPROTHR
2   PROALAPROTHRGLYTHRVALALAALAALA
3   VALTHRASPTHRGLNVALSERLYSASPASN
4   LYSPHEASPASPTHRLYSTHRLEUASNASN
5   ALAGLYALAASNGLYSERLEUSERASNSER
6   LYSGLYASNLEUGLYALAASNILEALAVAL
7   GLYSERGLYASNGLNGLNASPASNALAALA
8   ALAILETHRSERSERALAGLYASPALAALA
9   THRVALPHEALAVALALAASPILETYRGLN
10   GLNSERLYSASPASNLYSPHETHRASNLYS
11   GLYTHRGLNASNASNALALEULEUASNASN
12   SERALAASNASNSERSERGLYASNVALGLY
13   VALASNVALALAALAGLYGLNGLYASNGLN
14   GLNLYSASNASNLEUALAILEVALTHRALA
15   ASNGLYLYSASNVALALAALAALASERASN
16   THRGLUGLNVALSERLEUASPASNHISPHE
17   LEUASNGLUALASERSERLYSHISSERTYR
18   LYSPROGLNTYRVALVALASNASNALAGLY
19   LEULEUASNSERALAASNASNALASERGLY
20   ASNILEGLYVALASNVALALAALAGLYALA
21   GLYASNGLNGLNSERASNTHRLEUTHRLEU
22   GLYSERGLYCYSTHRVALCYSALAALAGLY
23   THRGLYSERLYSLEUALAPHELEUGLUHIS
24   HISHISHISHISHIS

Samples:

sample_1: FapC, [U-98% 13C; U-98% 15N], 0.2 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks