BMRB Entry 51538

Name: Backbone assignment for Functional Amyloid in pseudomonas FapB
Published: 2023-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51538

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone assignment for Functional Amyloid in pseudomonas FapB

Deposition date:

2022-08-01

Original release date:

2023-06-30

Authors:

Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Matthews, Steve

Citation:

Citation: Rasmussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC" J. Mol. Biol. 435, 167878-167878 (2023).
PubMed: 36368411

Assembly members:

Assembly members:
entity_1, polymer, 174 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSHHHHHHGTDSNNQALIDN AGKQYTGVLSVNQAAGNQHQ QINSRAISLGGQASDALIQK LDGKVDPSLNASAAIQGSAF SNGNGILGVNQSAGANNQMI NAVRISTGPQSVDDNVLSQQ NMTLLPDSRSPSTTGSRQVV TSDQAFTGSRGVVQVNQSAG VGNRMANTLGVTIK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	293
15N chemical shifts	158
1H chemical shifts	158

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FapB	1

Entities:

Entity 1, FapB 174 residues - Formula weight is not available

1

MET

SER

HIS

GLY

THR

2

ASP

SER

ASN

GLN

ALA

LEU

ILE

ASP

ASN

3

ALA

GLY

LYS

GLN

TYR

THR

GLY

VAL

LEU

SER

4

VAL

ASN

GLN

ALA

GLY

ASN

GLN

HIS

GLN

5

GLN

ILE

ASN

SER

ARG

ALA

ILE

SER

LEU

GLY

6

GLY

GLN

ALA

SER

ASP

ALA

LEU

ILE

GLN

LYS

7

LEU

ASP

GLY

LYS

VAL

ASP

PRO

SER

LEU

ASN

8

ALA

SER

ALA

ILE

GLN

GLY

SER

ALA

PHE

9

SER

ASN

GLY

ASN

GLY

ILE

LEU

GLY

VAL

ASN

10

GLN

SER

ALA

GLY

ALA

ASN

GLN

MET

ILE

11

ASN

ALA

VAL

ARG

ILE

SER

THR

GLY

PRO

GLN

12

SER

VAL

ASP

ASN

VAL

LEU

SER

GLN

13

ASN

MET

THR

LEU

PRO

ASP

SER

ARG

SER

14

PRO

SER

THR

GLY

SER

ARG

GLN

VAL

15

THR

SER

ASP

GLN

ALA

PHE

THR

GLY

SER

ARG

16

GLY

VAL

GLN

VAL

ASN

GLN

SER

ALA

GLY

17

VAL

GLY

ASN

ARG

MET

ALA

ASN

THR

LEU

GLY

18

VAL

THR

ILE

LYS

Samples:

sample_1: FapB, [U-98% 13C; U-98% 15N], 0.2 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks